摘要
目的建立依折麦布含量测定方法。方法采用高效液相色谱祛，以Agilent SB-C18（5μm，150mm×4．6mm）为色谱柱，以0．05mol／L磷酸二氢钾溶液-乙腈-四氢呋喃（65：25：10）为流动相；检测波长为232nm，柱温为25℃，流速为1．0mL／min。结果含量测定方法学研究表明，该方法专属性好；依折麦布在40．23-402．30μg／mL的浓度范围内与其峰响应值呈良好的线性关系，平均回收率为98．8％（RSD=0.3％）。结论本方法测定结果准确，精密度高，耐用性良好，可以作为依折麦布的含量测定方法。
Objective To establish the method for determination of Ezetimibe in Ezetimibe tablets, Methods The assay involved an isocratic elution of ezetimibe in a Agilent SB-C18 column （ 5μm,150mm × 4.6mm ） ,using a mobile phase composition of 0.05mol/L potassium dihydrogen phosphate and acetonitrile and tetrahydrofuran（65 ： 25：10）;The determination wavelength was 232 nm,column temperature was 25℃ and the flow rate was 1.0mL/min, Results After the Study on methodology for determination,It Indicated that the specificity of method was good;andezetimibe had a good linear range of 40.23-402.30μg/mL with a mean recovery of 98.8%（RSD=0.3%）, Conclusion The assay showed an accurate result,high precision and good durability,which can used as a method for determination of Ezetimibe in Ezetimibe Tablets.
出处
《中国医药科学》
2014年第12期108-111,共4页
China Medicine And Pharmacy
