摘要
目的将高表达血管生长素(ANG)的自体骨髓基质细胞移植于慢性心肌缺血猪模型,观察移植后血管新生程度及对心功能的影响。方法建立猪慢性缺血性心脏病动物模型,随机分为3组:转染ANG重组腺病毒载体(Ad-ANG)的自体骨髓基质细胞移植组(组Ⅰ,n=11),单纯自体骨髓基质细胞移植组(组Ⅱ,n=10),注射无血清DMEM培养液的对照组(组Ⅲ,n=10)。体外培养自体骨髓基质细胞,利用Ad-ANG转染培养细胞,用CM-DiI标记细胞,移植于模型猪心脏的缺血区域。观察指标包括:冠状动脉(冠脉)造影Rentrop分数及心脏射血分数、梗死区百分数、荧光显微镜下标记细胞、血管数量及ANG的表达和分泌。结果与组Ⅱ及组Ⅲ相比,组Ⅰ冠脉造影Rentrop分数、心脏射血分数增高;缺血心肌区新生血管计数增高,可见大量CM-DiI标记的移植成活细胞,梗死区面积较小,ANG蛋白表达增高,差异有统计学意义(P<0.05或P<0.01)。结论高表达ANG的自体骨髓基质细胞移植于慢性缺血心肌,移植细胞成活率高,能明显改善缺血心肌局部血运,改善心功能。
Objective To investigate the effect of autologous angiogenin（ANG）-overexpressing marrow stromal cells transplantation on the neoangiogenesis and cardiac function in a porcine model of chronic ischemia. Methods Porcine models of chronic ischemia were established and were randomized into the following 3 groups： autologous marrow stromal cells transfected with Ad-ANG （Group I , n= Ⅱ）, autologous marrow stromal cells without transfection （Group Ⅱ , n = 10）, and controls injected with serum-free DMEM medium （Group Ⅲ, n= 10）. The autologous marrow stromal cells were cultured and were transfected with Ad-ANG, and the cells were labeled by CM-DiI. The cells were transplanted to the ischemia site of porcine heart. The parameters we observed included Rentrop score, ejection fraction, percentage of infraeted area, CM-DiI labeled cells under fluorescence microscope, number of blood vessels and expression of ANG protein. Results Compared with group Ⅱ and Ⅲ , Group I had significantly increased Rentrop score, ejection fraction, numbers of blood vessels in the infarcted areas, CM- DiI labeled cells, and ANG protein expression, but significantly decreased infarcted area （P 〈 0. 05 or P 〈 0. 01 ）. Conclusion Transplantation of autologous angiogenin-overexpressing marrow stromal cells can yield a high survival rate of cells, greatly improving the blood supply of the infarcted cardiac area and cardiac function in porcine model.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2014年第6期586-591,共6页
Academic Journal of Second Military Medical University
基金
福建省医学创新基金(2012-CX-5)~~
