摘要
目的:研究当归种质资源的遗传多样性,探讨当归不同种质之间的亲缘关系,为当归的种质资源收集保存及良种选育提供科学的理论依据。方法:运用扩增片段长度多态性(AFLP)技术,对12个当归居群117个个体进行遗传多样性研究,用NTSYSpc 2.11软件进行聚类分析并构建遗传系统进化树。结果:从64对引物中筛选得到8对AFLP引物组合,共扩增出815个DNA条带,多态性条带为812个,多态位点百分率99.63%;通过构建系统进化树,野生种质和栽培种质当归以及不同表型当归各自聚类。结论:AFLP分子标记表明当归遗传多样性比较丰富,不同种群分化较大,紫茎和绿茎两种表型当归可能具不同基因型,为当归种质资源的合理利用和新品种选育提供了分子水平的理论依据。
Objective： To explore the genetic diversity and relationship of different germplasm of Angelica sinensis.Methods： Amplified fragment length polymorphism（ AFLP） markers were developed to analyze genetic polymorphism in 12 populations 117 samples of Angelica sinensis.The amplified fragments were used for cluster analysis among the different germplasm of Angelica sinensis and to construct the genetic phylogenetic tree with NTSYSpc 2.11 software.Results： Eight primer combinations selected from 64 primer combinations were used for amplification and a total of 815 fragments were obtained.Analysis identified 812 polymorphic fragments,accounting for 99.63% of the total detected fragments.Different phenotypes and germplasm of Angelica sinensis could be divided by genetic phylogenetic tree analysis.Conclusion： AFLP molecular markers can indicate the significant polymorphism and genetic diversity among germplasm resources of Angelica sinensis.The cultivated purple-stemmed and green-stemmed phenotypes of Angelica sinensis may have different genotypes.The results can provide theoretical evidence for reasonable utilization and breeding new cultivar in molecular level.
出处
《中药材》
CAS
CSCD
北大核心
2014年第4期572-575,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(31300272)
广州市科技局项目(2013KP020)
关键词
当归
种质资源
AFLP
遗传多样性
Angelica sinensis（Oliv.） Diels Germplasm resources Amplified fragment length polymorphism（AFLP） Genetic diversity
