摘要
经点滴法和平板法检测 ,放线菌噬菌体ΦC31及大部分衍生噬菌体能感染南昌链霉菌 ,但这些噬菌体的DNA却不能转染南昌链霉菌 .通过比较来自不同宿主的KC5 15噬菌体悬液对变铅青链霉菌和南昌链霉菌的侵染效率 ,发现南昌链霉菌对ΦC31及衍生噬菌体具有很强的限制性 .Southern杂交实验表明 ,ΦC31衍生噬菌体KC30 1(C+ attP+ )可以整合到南昌链霉菌NS - 32 - 2 6的染色体上形成溶源 ,其溶源菌自发释放KC30 1,但热激诱导后并没有提高释放频率 .通过脉冲电泳技术和Southern杂交的方法定位了KC30 1在南昌链霉菌NS - 32 - 2 6染色体上形成溶源的附着位点 (attB) .这些结果均有利于利用以ΦC31为基础的载体对南昌链霉菌进行分子生物学的研究 .
Actinophage ΦC31 and most of its derivatives can infect S. nanchangensis NS-32-26 but not transfect. Comparison of e.o.p between NS-32-26 and S.lividans ZX1 by phage infection indicates S. nanchangesis has restriction. Southern hybridization indicates that KC301 derived ΦC31 can lysogenize S. nanchangensis by integrating into its chromosome. Phage production was observed after incubation of KC301 lysogenic spores. In addition, attB site on the chromosome of S. nanchangensis NS-32-26 has been located by the use of pulse-field gel electrophoresis and Southern hybridization. These information will be helpful for studies of S. nanchangensis molecular biology based on ΦC31 vector.
出处
《湖北大学学报（自然科学版）》
CAS
2001年第1期82-86,共5页
Journal of Hubei University：Natural Science
