摘要
目的 比较牛牙与人牙两种不同釉质样品体外形成早期人工龋损的病理学特征,寻找更接近人牙龋损的实验标本,以便于龋病病因及防治的研究.方法 在体外人牙釉质与牛牙釉质上制备人工早期釉质龋损,采用体视显微镜、透射光显微镜、偏振光显微镜和扫描电镜观察人工龋的病理学变化.结果 经脱矿后,两种牙釉质样本实验开窗区表面完整,呈白垩色不透明状;在人牙釉质与牛牙釉质上制备的人工龋损都具有完整的表层和表层下脱矿,但牛牙釉质脱矿深度大于人牙釉质;牙釉质表面可清楚见到釉柱的中心溶解破坏,可见较清晰的、成束的晶体颗粒,牛牙与人牙略有不同,凹坑略大于人牙,晶体颗粒较人牙的稍粗.结论 体外脱矿系统在牛牙釉质制备的人工龋损病理学特点类似于人牙釉质,与早期自然龋损相似,故与人牙具有相似化学组成的牛牙很适合代替人牙用于龋病的研究.
Objective To pathologically evaluate the production of artificial carious lesion in bovine and human enamel in vitro and to look for teeth from other species to provide quantities of standardized material for caries disease research.Methods The Lesions in bovine and human enamel were produced by 3-day exposure to a partially saturated acidic buffer solution.The histopathology of these enamel specimens were observed and compared by microscope,polarized light microscopy and scanning electron microscope.Results All specimens in a partially saturated acidic buffer solution were observed as a white opaque spot and could morphologically form subsurface demineralization with a relatively intact surface layer,which was the same as the early natural enamel caries,except that the lesions depth was deeper than those in human teeth.Subsurface lesions in bovine enamel and human enamel show an irregular and eroded surface with openings.The crystallites are short,have club-like endings and irregular shape,but The crystallites in bovine enamel are less densely packed,more Larger in size than those in human enamel.Conclusion Lesions in bovine teeth were pathologically similar to those in human teeth,so bovine enamel is considered to be a suitable surrogate for human enamel in caries research.
出处
《实验动物与比较医学》
CAS
2014年第3期176-180,共5页
Laboratory Animal and Comparative Medicine
基金
国家自然科学基金资助项目（No.81141119
No.u1204825）
河南省科技厅重点科技攻关（No.122102310243）
河南省高等学校青年骨干教师资助计划资助（201124）
关键词
人牙釉质
牛牙釉质
人工龋
病理学
Human enamel
Bovine enamel
Artificial carious lesion
Pathology
