摘要
研究了卤素 (F ,Cl,Br ,I)与喹诺酮类药物司帕沙星反应产物的荧光特性 ,发现在酸性介质中 ,亚硝酸可氧化司帕沙星继而被卤素取代 ,所得产物具有强烈的荧光发射 ,其荧光强度随着卤素原子半径的增大呈现规律的增强效应。司帕沙星卤代物荧光较司帕沙星强 5 8～ 15 1倍。据此建立了卤素增强荧光光谱测定司帕沙星的新方法。该方法用于直接测定尿液中司帕沙星的含量 ,结果令人满意。本文还对司帕沙星的荧光增敏机理进行了探讨。
The fluorescence characteristics of reaction products of sparfloxacin with halide (F, Cl, Br, I) have been studied. It was found that sparfloxacin was oxidized by nitrous acid then reacted with halide further to formstrong fluorescence substances in acid media. Their fluorescence intensities enhanced with aggrandizing of atom radii of halides, which could emit the strong fluorescence 58 similar to 151 fold more than that of sparfloxacin itself. Thus a new sensitive method for the direct determination of sparfloxacin in human urine by derivative-synchronous fluorescence was presented. A plausible mehanism was proposed to explin this behavior.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2001年第3期249-252,共4页
Chinese Journal of Analytical Chemistry
