摘要
目的：通过检测桥本甲状腺炎（HT）患者外周血单个核细胞（PBMC）Th17特异性转录因子等的mRNA水平及观察患者B超表现，并与健康者对照，为HT的诊断提供新思路。方法：用超声检查观察74例确诊HT患者甲状腺形态及内部回声改变，测量甲状腺各径线大小，彩色多普勒血流成像（CDFI）技术观察其血流信号分布状况；应用实时荧光定量PCR（QRT-PCR）技术检测患者及健康对照者PBMC中白细胞介素17（IL-17）和视黄醛酸相关孤儿核受体（ROR,yt）的mRNA水平。结果：92％的HT患者甲状腺呈弥漫性增大，以前后径及峡部增厚为特征，内部回声以弥漫性减低为主，血流信号多表现为轻～中度增多。HT患者PBMC中IL-17和RORγt mRNA的表达水平均明显高于健康对照者（P〈0．01）。结论：甲状腺B超检查联合外周血Th17及RORγt检测，可以提高HT的检出率。
Objective： To provide new ideas for diagnosis and treatment in patients with Hashimoto＇s thyroiditis （HT） ,through detecting the B ultrasonic manifestation and the mRNA level of specifie transcription faetor of Thl7 cell in the peripheral blood of the patients with HT and their relationship. Methods： We studied 74 patients definitely diagnosed HT and the normal control group. Through uhrasongraphy, the shape and the changes of echogenicity were obseved in the patients;also the size of thyroid measured was measured;blood flow distribution was detectedusing Color Doppler. At the same time, we deteetd the mRNA level of IL- 17 and RORγt in the peripheral blood mononuclear cells of the patients through real-time quantitative fluorescence. Results： The size of thyroid of the 92 percentage patients with HT was diffusely increased, and much larger than those in the normal eontrol group characterized by thickening of anteroposterior and isthmus. Their eehogenicity was primarily diffusely reduced. Their blood signal manifested mild-to-moderate increasing. The mRNA level of the IL-17 and RORγt in PBMC of patients with HT was obviously higher than the normal control group （P 〈 0. 001 ）. Conclusion： B ultrasond combined with Thl7 and RORγt to detect HT improves detection rate of HT.
出处
《现代医学》
2014年第6期611-615,共5页
Modern Medical Journal
