摘要
利用Ｌａ Ｐｕｒｐｌｅ（Ｓ． ｏｆｆｉｃｉｎａｒｕｍ Ｌ．） Ｘ Ｍｏｌ ５８２９（Ｓ．ｒｏｂｕｓｔｕｍ Ｂｒａｎｄｓ ａｎｄ Ｊｅｓｗｉｅｔ ｅｘＧｒａｓｓｅｌ）杂交一代群体获得的ＲＦＬＰ和ＲＡＰＤ分子标记信息，用区间定位法（ ｉｎｔｅｒｖａｌ ｍａｐｐｉｎｇ）和单标记分析（ｐｏｉｎｔ ａｎａｌｙｓｉｓ）法测定控制转光度（％）、小区蔗茎产量、纤维分（％）和抽穗茎率的ＱＴＬ。选用与转光度和茎产量显著关联（Ｆ测验Ｐ＜０．０１或区间定位中ＬＯＤ＞２）的分子标记进一步对标记辅助选择的效果进行分析。对任一性状而言，与之显著关联的标记都含有正效应的和负效应的两类标记。由于单标记分析法可测定出未被归类到连锁群、但与ＱＴＬ有关联的标记，故其可测定到的显著性标记要比区间定位法的多。分析结果表明，控制转光度和茎产量的ＱＴＬ分散于多个连锁群中，而控制抽穗茎率的ＱＴＬ相对集中于较少的连锁群中。在利用分子标记进行性状选择时，增加用于辅助选择的标记数目将使入选群体的性状平均值增大，而使误选低值个体的可能性减小。在多标记辅助选择时，各个标记都选用正效应的状态（标记出现或缺失）所得到的标记组合将可选得性状平均值最大的入选群体。
Quantitative trait loci (QTLs) controlling %pol, cane weight per plot, % fiber and %tasseling stalks in sugarcane were detected by interval mapping and point analysis (one-way ANOVA) using single-dose RFLP and RAPD marker information from a F1 population derived from La Purple (S. officinarum L. ) × Mol 5829 (S. robustum Brands and Jeswiet ex Grassel). Significant markers (P<0. 01 in F-test or LOD> 2) linked to Pol and Weight were selected for the further study on selection effects of marker-Assisted selection. For each trait, significant markers included both positive and negative ones. Point analysis identified more significant markers than interval mapping analysis, since it can detect unlinked markers associated with QTLs. The results indicated that the QTLs controlling both Pol and Weight were located in many linkage groups, while the QTLs controlling Tasseling concentrated on relatively fewer linkage groups. The results indicated that increasing the number of markers used for marker-assisted selection will result in higher mean trait value of the selected population, and lower risk of selecting lower trait value individuals in the mixed breeding population. The marker combination with each marker in its higher trait value marker class appeared to be best choice for multiple marker assisted selection, since it led to highest mean trait value of the selected population.
出处
《甘蔗糖业》
2001年第1期1-12,共12页
Sugarcane and Canesugar
