摘要
对云纹石斑鱼和赤点石斑鱼及其正反杂交子代的3种线粒体基因（COⅠ、16S rDNA、Cyt b）和核基因Tmo-4c4进行序列分析,其中16S rDNA序列中有明显的插入缺失位点,而其他3个基因序列无插入缺失变异。在12个分析样本中,COⅠ同源序列（387 bp）中共检测到41个核苷酸多态性位点,16S rDNA（529 bp）中有21个核苷酸多态位点,Cyt b（383bp）中有49个核苷酸多态位点。Tmo-4c4（467 bp）有8个核苷酸多态位点。序列差异分析和遗传距离比较结果显示,正反杂交子一代的3个线粒体基因序列与母本基因序列的同源性都为100%,与父本的基因序列同源性分别为COⅠ：90%和89.4%;16S rDNA：96%和96%;Cyt b：87%和87.2%。核基因Tmo-4c4正反交子一代与母本的序列同源性为98.9%~99.6%之间,与父本的同源性为98.7%~99.4%之间,没有明显的遗传差异。以上结果表明了云纹石斑鱼和赤点石斑鱼杂交子一代在3种线粒体基因上严格按照母性遗传的规律,而核基因Tmo-4c4没有明显的遗传偏向性。
We analysed the sequences of three mitochondrial DNA（ COⅠ）,16S rDNA,cytochrome oxidase b） and nuclear gene Tmo-4c4 to identify the phylogenetic relationship among the grouper hybrids and their parents（ Epinephelus moara ♀ and Epinephelus akaara ♂; Epinephelus moara ♂ and Epinephelus akaara ♀）. Among the four sequence fragments there was insertion deletion 16S rDNA but no insertion deletion in other three gene fragments which all belong to coding gene. As shown in results,in 12 sequences there were 4 haplotypes and 41 nucleotide polymorphic loci in COⅠ gene fragments（ 387 bp）,2 haplotypes and 21 nucleotide polymorphic loci in 16S rDNA gene fragments（ 529 bp）,2 haplotypes and 49 nucleotide polymorphic loci in Cyt b gene fragments（ 383 bp）,and 6 haplotypes and 8 nucleotide polymorphic loci in 16S rDNA gene fragments（ 467 bp）. Sequence divergences and genetic distance analysis showed that three mitochondrial gene sequences in the two crossing hybridized generations all showed 100% similarities respectively with the fragments of the female parents,but there were only 90% and 89. 4% similarity in CO Ⅰ,96% and 96% similarity in 16S rDNA and 87% and 87. 2% similarity in Cyt b with their male parents. In nuclear gene Tmo-4c4 there were 98. 9% to 99. 6% similarity with their female parents and 98. 7% to 99. 4% similarity with their male parents. The results indicated that there was no remarkable genetic difference. All above results imply that the two crossing grouper hybrids inherited from their female parents in three mitochondrial genes and there is no remarkable genetic difference in nuclear gene Tmo-4c4.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2014年第3期351-358,共8页
Journal of Shanghai Ocean University
基金
福建省石斑鱼种业创新与产业化工程建设[闽种石斑鱼(004号)]
关键词
云纹石斑鱼
赤点石斑鱼
杂交子代
线粒体基因
核基因
遗传变异
Epinephelus moara
Epinephelus akaara
hybridized generations
mitochondrial gene
nucleargene
genetic variation
