摘要
为探讨微型土壤动物在人工林生态系统中的功能及其对环境变化的响应,利用18S rDNA宏基因组比较了湖南省攸县不同发育阶段(3、13、26年)以及不同栽培代数(1、2、3代)杉木人工林微型土壤动物的群落多样性及结构.结果表明:焦磷酸测序共得到94922条优质序列,平均长度为436 bp.杉木人工林微型土壤动物主要类群包括:脊索动物门60.8%、环节动物门24.0%、节肢动物门7.4%、线虫动物门3.6%、轮形动物门1.5%和扁形动物门1.2%.5块样地的ACE指数与Shannon指数具有显著性差异,其中第1代13年生杉木林的ACE指数(712.33)、Chao1指数(500.00)、Shannon指数(2.66)均最低,Simpson指数(0.26)最高,表明第1代13年生杉木林微型土壤动物多样性最低.相关分析发现,速效钾含量与ACE指数和Chao1指数之间存在显著负相关,而Shannon指数、Simpson指数与所测土壤理化性质之间无显著相关关系.各样地Jaccard相似性系数均<0.4,样地间微型土壤动物群落结构差异明显.
In order to study the function of soil microfauna and its responses to environmental chan- ges, we used metagenome analyses of the 18S rDNA gene region to identify differences in microfau- na diversity and community structure among fifteen soil samples belonging to five different Cunning- hamia lanceolate plantations. The plantations were located in Youxian County, Hunan Province in central China. The trees in these plantations were of different ages （3, 13, and 26 years） and be- longed to different ecological successions （first, second, and third successions）. The total dataset comprised 94922 high quality sequences with an average length of 436 bp. The dominant taxonomic groups across all samples were Chordata, Annelida, Arthropoda, Nematoda, Rotifera and Platyhel- minthes with each accounting for 60. 8 % , 24.0%, 7.4%, 3.6%, 1.5% and 1.2% of the se- quences, respectively. There were significant differences in ACE index and Shannon index among the five plantations. The lowest diversity of soil microfauna was in the 13-year old plantation of the first ecological succession. The correlation analysis showed that both ACE and available potassium concentration were negatively correlated to the Chaol index. However, there were no significant correlations between the Shannon, Simpson indices and the physical-chemical properties of soil. Overall, the Jaccard＇ s similarity coefficient was less than 0.4 among samples at each site, and sig- nificant differences were found among plantations.
出处
《应用生态学报》
CAS
CSCD
北大核心
2014年第6期1661-1668,共8页
Chinese Journal of Applied Ecology
基金
国家林业局重点项目(2012-01)
国家林业公益性行业科研专项(201004014)资助
