摘要
目的观察慢性丙型病毒性肝炎患者抗病毒治疗前后外周血中NK细胞、NK细胞亚群百分率及活化因子CD100表达水平变化。方法流式细胞术检测慢性丙型肝炎病毒(HCV)感染者外周血中NK细胞、NK细胞亚群频率及膜型CD100表达水平;ELISA检测血清中可溶性CD100水平;采用Spearman相关检验分析CD100与谷丙转氨酶(ALT)、HCV-RNA之间的相关性。结果与健康对照组相比,慢性HCV感染患者CD56dimNK亚群百分率降低,而CD56neg亚群比率升高(P<0.05);抗病毒治疗后获得早期病毒学应答(EVR)者,CD56bright亚群百分率升高,CD56neg亚群比率显著降低(P<0.05);停药后获得持续性病毒学应答(SVR)者,总NK细胞及亚群频率恢复正常。与健康对照组相比,慢性HCV感染患者血清中sCD100水平显著降低(P<0.05);膜型CD100表达轻度降低,但无统计学差异;经抗病毒治疗获得EVR者,sCD100及膜型CD100水平均显著升高(P<0.05),获得SVR者又降至正常水平。Spearman相关性分析发现,NK细胞CD100表达与ALT水平呈正相关,与HCV-RNA滴度呈负相关(P<0.05)。结论 CD100表达降低与HCV持续感染有一定关系,并参与NK细胞对HCV清除。
Objective To investigate the changes of total natural killer （NK） cell number and its subset proportions and the expression levels of activation factor CD100 on NK cells in chronically hepatitis C virus （HCV） infected patients before and after initiation of antiviral treatment. Methods The frequencies of total NK cells and their subsets as well as CD100 expression on NK cells in HCV infected patients were detected by flow cytometry. The level of soluble CD100 （sCD100） in serum was measured by ELISA. The correlations of CD100 with alanine aminotransferase （ALT） and HCV-RNA were analyzed using Spearman correlation test. Results The proportion of CD56dim NK cell subset decreased, whereas CD56neg subset increased in chronic HCV infected patients as compared with healthy controls （ P 〈 0.05）. The proportion of CD55bright NK cells was elevated and that of CD56neg NK subsets was reduced in HCV infected patients with early virological response （EVR） （P 〈 0.05）; however, total NK cells and their subsets returned to the normal in patients with sustained virological response （SVR） after the initiation of antiviral treatment. We also observed that sCD100 level declined significantly （P 〈 0.05）, whereas CDI00 expression on NK cells slightly decreased as compared with healthy controls, but the difference was not statistically significant. The levels of sCD100 and CD100 expression on NK ceils were markedly up-regulated in EVR patients （P 〈0.05） and dropped to the normal in SVR patients. Spearman correlation analysis showed that CD100 expression was positively related to ALT level and negatively associated with HCV-RNA titer in HCV infected patients （ P 〈 0.05）. Conclusion Decreased CD100 expression may be associated with viral persistence. As one of immune molecules regulated by IFN-α, CD100 seems to be involved in HCV clearance by NK cells.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2014年第8期856-860,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81300314
81273218)
