摘要
目的：建立康普瑞丁A4磷酸酯（CA4P）聚合物囊泡中CA4P的含量测定方法。方法：采用反相高效液相色谱法。色谱柱为C18，流动相为乙腈-甲醇-0．02mol／L乙酸铵（10：40：50），流速为1．0ml／min，检测波长为288nm。结果：CA4P检测质量浓度线性范围为2．1～42μg／ml（r=0．9999），平均回收率为96．72％（RSD=1．61％，n=3），检测限与定量限分别为0．21、0．70μg／ml。结论：本方法简便、准确度好，可有效控制CA4P聚合物囊泡中CA4P的含量。
OBJECTIVE： To establish the method for the content determination of combertastatin A4 phosphate （CA4P） poly- mersomes vescia. METHODS： RP-HPLC method was adopted. The determination was performed on C18 column with mobile phase consisted of acetonitrile-methanol-0.02 mol/L ammonium acetate （10：40：50） at the flow rate of 1.0 ml/min. The detection wave- length was set at 288 nm. RESULTS： The linear range of CA4P was 2.1-42 μg/ml （r=0.999 9） with an average recovery of 96.72% （RSD=1.61%, n=3）. The limits of detection and quantification were 0.21μg/ml and 0.70 μg/ml. CONCLUSIONS： The method is simple and accurate, and can be used to effectively control the content of CA4P in polymersomes vescia.
出处
《中国药房》
CAS
CSCD
2014年第25期2381-2383,共3页
China Pharmacy
