摘要
3-苯氧基苯甲酸是绝大多数拟除虫菊酯类农药的降解中间产物。以对3-苯氧基苯甲酸具有较强耐受性和较高降解能力的米曲霉M-4为实验菌株,考察了不同培养条件对该菌株产3-苯氧基苯甲酸降解酶活力的影响,并且分析了该降解酶的酶学性质。结果表明,当15 g固体培养基的初始pH为6.5、3-苯氧基苯甲酸浓度为150μg/g、接种量为2.25 mL(孢子悬浮液浓度约为107cfu/mL)时,30℃培养120 h,提取得到的3-苯氧基苯甲酸降解酶的活力最高,为53.42 U;该降解酶作用的最适pH和最适温度分别为7.0和30℃,酶的稳定pH和稳定温度范围分别为5.5～7.0和5～30℃。
3-phenoxybenzoic acid （3-PBA） is a common degradation intermediate product of pyrethroid insecticides. In this study, the strain Aspergillus oryzae M-4 with high tolerance and good degradation ability to 3-PBA was used. The effects of different incubation conditions on the activity of 3-PBA degradation enzyme produced by the strain M-4 were investigated. And properties of the 3-PBA degradation enzyme under different pH and temperature conditions were studied. It was found that the maximum activity of 3-PBA degradation enzyme was obtained as 53.42 U when the initial pH of medium, 3-PBA concentration, inoculation volume, incubation temperature and time were 6.5, 150 μg/g, 2.25 mL （spore suspension concentration was 10^7 cfu/mL）, 30 ℃ and 120 h, respectively. The optimum pH and temperature of the enzyme were 7.0 and 30 ℃, respectively, and the pH and temperature range for the enzyme with good stability were 5.5-7.0 and 5-30 ℃, respectively.
出处
《食品科技》
CAS
北大核心
2014年第6期17-21,共5页
Food Science and Technology
基金
国家自然科学基金项目(31371775)
关键词
3-苯氧基苯甲酸
降解酶
米曲霉
产酶条件
酶学性质
3-phenoxybenzoic acid
degradation enzyme
Aspergillus oryzae
enzyme productioncondition
enzymatic property
