摘要
目的 探讨不同时段哺乳期母爱剥夺对子代小鼠成年后抑郁情绪的影响.方法 新生SD大鼠随机分为5个不同时段母爱剥夺组（n=5×5）和对照组（n=7）.母爱剥夺组在不同的年龄阶段进行连续5d母爱剥夺（出生当天为p0,出生后第5天为p5,出生后第10天为p10,出生后第15天为p15,出生后第20天为p20）,剥夺期间每天进行6h的母婴分离.当这些大鼠长到10周通过强迫性游泳训练、毛发梳理、葡萄糖消耗实验来评定抑郁行为,同时通过高效液相色谱法测定大脑中儿茶酚胺类神经调质的改变.结果 母爱剥夺组大鼠表现出明显的主动游泳降低,静止漂浮时间从对照组的（58.5±7.5）s,增加到p0：（75.6±9.4） s,p5：（150.4±12.5） s,p10：（174.5±14.3） s,p15：（155.4±11.4） s,p20：（112.4±10.3）s（均P＜0,01,n=5～7）.与对照组（0.87±0.1）比较,母爱剥夺组糖水消耗降低[p0（0.73±0.12）,p5（0.53±0.09）,p10（0.5±0.06）,p15（0.54±0.07）,p20（0.67±0.05）].毛发梳理得分从对照组的（4.5±0.6）分,降低到p0：（3.3±0.6）分,p5：（2.3±0.4）分,p10：（1.9±0.3）分,p15：（2.4±0.4）分,p20：（4.4±0.4）分.与对照组相比,母爱剥夺组大鼠脑内儿茶酚胺水平降低（分别是p0：（94.5±11.5）％;p5：（78.5±9.6）％;p10：（67.6±7.4）％;p15：（64.5±6.6）％;p20：（88.5±7.5）％,均P＜0.01,n=5～7）.结论 出生后哺乳期内10 d左右的母爱剥夺可以明显引起抑郁行为,导致大脑内儿茶酚胺类神经调质的改变.
Objective To investigate the effects of maternal deprivation at different time periods in rat pup development on depressions in adults.Methods Newborn SD rat pups were randomly divided into 5 maternal deprivation groups（n=5 in each group） and one control group （n=7）.The rat pups in the maternal deprivation groups were separated from their mother for 6 hours in one day,at p0,p5,p 10,p15,p20 respectively,lasting for five days.Then the rats will be screened for depression at the age of 10 weeks,using different behavioral tests,which included forced swim,coat score,sucrose preference test.Norepinephrine concentration in the cerebral spinal fluid was also measured through high performance liquid chromatography （HPLC）.Results Maternal deprivation for rat pups induced depression like behavior in adults.The floating time during forced swim test increased from（58.5 ±7.5） s in control group to（p0（75.6±9.4） s,p5（150.4±12.5） s,p10（174.5±14.3） s,p15（155.4±11.4）s,p20 （112.4±10.3） s respectively in maternal deprivation groups;and it was significant different among the groups（P＜ 0.01）.Compared with the control group（0.87±0.1）,the sucrose preference in maternal deprivation groups decreased（p0 （0.73±0.12）,p5 （0.53±0.09）,p10 （0.5±0.06）,p15 （0.54±0.07）,p20 （0.67±0.05））.And the coating scores in maternal deprivation groups decreased from 4.5±0.6 in control group to p0（3.3±0.6）,p5 （2.3±0.4）,p10 （1.9±0.3）,p15 （2.4±0.4）,p20 （4.4± 0.4）.Finally,maternal deprivation also induced low concentration of catecholamine in the cerebral spinal fluid compared with that in control group （p0 （94.5± 11.5） ％,p5 （78.5± 9.6） ％,p10（67.6±±7.4）％,p1S（64.5±6.6）％,p20（88.5±7.5）％,P＜0.01,n=5-7）.Conclusion Maternal deprivation at different lactation periods may induce depression behaviors in rat offspring,and also lead to lower concentration of catecholamine in the cerebral spinal fluid.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2014年第5期394-397,共4页
Chinese Journal of Behavioral Medicine and Brain Science
基金
江苏省特聘教授专项基金
南京中医药大学特聘教授专项基金
关键词
母爱剥夺
抑郁行为
儿茶酚胺
Maternal deprivation
Depression behavior
Catecholamine
