摘要
目的探讨密骨打老儿丸(打老儿丸加淫羊藿、骨碎补、补骨脂、菟丝子等)对去卵巢骨质疏松大鼠生化指标的影响。方法 72只SD雌性大鼠,随机分为6组:假手术组、模型组、维生素D3(2 mg/kg)组和密骨打老儿丸小、中、大剂量组。假手术组仅行假手术,其余5组分别行卵巢切除术。术后1周分别灌胃给予密骨打老儿丸和维生素D33个月。采用酶联免疫吸附法检测血清1,25-二羟基维生素D3(1,25(OH)2D3)水平。测定股骨骨密度,采用实时荧光RT-PCR测定第2腰椎和肾脏组织维生素D受体(VDR)mRNA表达。结果与假手术组相比,模型组股骨骨密度、血清和肾脏1,25(OH)2D3、腰椎和肾脏组织VDR mRNA表达均下降。与模型组比较,密骨打老儿丸中、大剂量组和维生素D3组均可使股骨骨密度、血清和肾脏1,25(OH)2D3、腰椎和肾脏组织VDR mRNA表达增加。结论密骨打老儿丸和维生素D3显著提高去卵巢大鼠腰椎和肾脏组织VDR mRNA表达,促进肠钙吸收与成骨细胞活性,增强骨质量。
AIM To explore the influence of Migu Dalao＇ er Pills （Epimedii Herba, Drynariae Rhizoma, Psoraleae Fructus, Cuscutae Semen, etc. ） on vitamin D receptor mRNA expression of the second lumbar vertebra and kidney tissue in rats with osteoporosis caused by ovariectomy. METHODS Seventy-two Sprague Dawley female rats were randomly divided into six groups： the sham group, the model group, alfacalcidol treated group, and Migu Dalao＇ er Pills treated groups of low 1.25 mL/kg , middle and high doses. The sham group was only sham operated and the other five groups were ovariectomized respectively. One week after ovariectomy, the rats were consecutively given Migu Dalao＇ er Pills and alfacalcidol by intragastric administration for three months. ELISA was used to determine 1,25 （OH）2 Dz contents of renal tissue and serum. The thighbone was taken out to de- termine bone mineral density （BMD）. Real-time fluor-PCR was used for quantitative analysis of the vitamin D receptor mRNA expression of second lumbar vertebra and renal tissue. RESULTS Compared with the sham group, BMD of femur, and 1,25 （OH） 2Dz level and the expression of vitamin D receptor mRNA significantly decreased in the model group. Compared with the model group, middle and high doses of Migu Dalao＇ er Pills groups and the alfacalcidol group showed increased BMD, 1,25 （OH）2D3 level, and vitamin D receptor mRNA expression. CONCLUSION Migu Dalao＇ er Pills could significantly stimulate vitamin D receptor mRNA expression to accelerate intestinal calcium absorption and osteoblast activity and reinforce the quality of bones.
出处
《中成药》
CAS
CSCD
北大核心
2014年第6期1133-1138,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目(81073074
30472200)
河北省自然科学基金项目(H2013206005)
河北省留学人员科技活动项目择优资助项目(200828)
