摘要
目的:观察人参皂苷Rg1对鼠胚成纤维细胞(MEF)向诱导多潜能干细胞(iPSCs)诱导转化效率的影响。方法:采用经典四因子的逆转录病毒载体感染,在细胞感染后3 d内在MEF培养基里加入人参皂苷Rg1(0、0.3、1、3、10μg/mL),进行iPSCs诱导及鉴定。结果:人参皂苷Rg1(1μg/mL)组可明显提高iPSCs的诱导效率,诱导效率为(0.0450±0.0019)%,人参皂苷Rg1(0μg/mL)组为(0.0100±0.0033)%,所获iPSCs经鉴定为阳性。结论:人参皂苷Rg1可能在提高小鼠iPSCs的诱导效率方面发挥一定作用。
ObjectiveTo observe the effects of Ginsenoside Rg1 on the induce efficiency of mouse induced pluripotent stem cells （iPSCs） from Mouse embryonic fibroblasts （MEF）. Methods： The cells were infected with classic retro-viruses vector expressing classic 4 factors, then Ginsenoside Rg1 （0 、0.3、1、3、10 μg/mL） was added to the MEF medium during the first 3 days after infection. Then the clones were identified. Results： Ginsenoside Rg1 （1 μg/mL）can obviously improve the induction efficiency of iPSCs [（0.0450±0.0019）% in the Ginsenoside Rg1group vs （0.0100±0.0033）% in the control group）]. The clones were positive. Conclusion： Ginsenoside Rg1 may facilitate the inducing efficiency of iPS cells.
出处
《神经损伤与功能重建》
2014年第3期184-186,共3页
Neural Injury and Functional Reconstruction
基金
国家自然科学基金(No.81271407)
中国博士后科学基金(No.20110490453)
