摘要
目的研究超抗原 SEA对外周血 T淋巴细胞的活化作用。方法用 SEA刺激人外周血 T淋巴细胞 ,观察细胞的 DNA合成、形态、IL - 2的产生、细胞表型以及凋亡情况。结果 SEA对 T淋巴细胞的促增殖作用在浓度为 10 0 ng/m L最强 ,在第 2、3d达高峰 ;首次或多次刺激不改变 T淋巴细胞的 CD4+ CD8+ 表型 ;首次刺激 2 4h内未见明显凋亡 ;但再次刺激 2 4h内即有明显凋亡 ;加入 rh IL - 2 2 d凋亡现象消失。结论超抗原 SEA对 T淋巴细胞的促增殖作用与其浓度、作用时间有关 ,并且SEA首次或多次刺激对 CD4+ T淋巴细胞和 CD8+
ObjectiveTo investigate the activation of human T cells with superantigen SEA. MethodsT cells proliferation, IL 2 production, DNA synthesis, cell phenotype and apoptosis induced by the SEA in the first or restimulation were detected. Results It was shown that 100 ng/mL was the optimal activation concentration, IL 2 production arrived at top level in the third day, SEA activated CD4 + and CD8 +T with the same degree, T cells start apoptosis in response to SEA restimulation within 24 hours and apoptosis disappeared through addition of rhIL 2. Conclusions There were correlation between activation and SEA concentration or stimulation period; SEA activated both CD4 + and CD8 +T cells without change the cell phenotype.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第2期85-87,104,共4页
Immunological Journal
基金
全军"九五"医药卫生科研重点项目基金!(96 Z0 39)资助项目
