摘要
目的 :开发生产γ 亚麻酸 (GLA)新的微生物资源。方法 :研究温度、时间、碳源和氮源对雅致枝霉(Thamnidiumelegans)诱变株TE7 15的菌体生长、油脂积累及GLA合成的影响。结果 :确定了TE7 15菌株适宜的摇瓶发酵培养条件 ,其菌体生物量达到 19.0 4g/L ,GLA产量达到 10 79.95mg/L。 结论
Purpose:The aim is to exploit a new microoganism resource for γ linolenic acid(GLA) production.Methods: The effects on fermentation conditions,such as temperature,fermentation time,carbon source and nitrogen source on cell growth and lipid formation of Thamnidium elegans TE7 15 strain were studied.Results:The optimum culture medium and culture conditions were established,and the dry cell weight and γ linolenic acid yield were 19.04 g/L and 1 079.95 mg/L under the conditionrespectively.Conclusion:The strain may be used for the industrial production of GLA.
出处
《中国生化药物杂志》
CAS
CSCD
2000年第6期277-279,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
雅致枝霉
发酵
Γ-亚麻酸
实验
Thamnidium elegans, Fermentation, γ linolenic acid