摘要
目的:从肠道菌群探讨菊苣提取物干预腹型肥胖大鼠的药效机制。方法:雄性SD大鼠随机分为5组,即正常组、模型组、菊苣高、低剂量组和非诺贝特组。正常组给予去离子水,其他组给予果糖水并给予菊苣、非诺贝特治疗,生化法检测甘油三酯、总胆固醇、低密度脂蛋白、高密度脂蛋白,测量体重、腹围,实时荧光定量PCR微观观察肠道菌群的变化。结果:与正常组相比,模型大鼠甘油三酯水平、腹围明显升高(P<0.05),体重、高密度脂蛋白有所增加但未见明显变化,大肠杆菌、乳酸菌数量明显增加;与模型组相比,菊苣提取物高、低剂量组及非诺贝特组可以显著降低甘油三酯水平(P<0.05)、降低大肠杆菌及乳酸菌的数量、增加双歧杆菌的数量;非诺贝特组可显著降低总胆固醇和高密度脂蛋白的水平。结论:菊苣治疗腹型肥胖效果显著,其干预腹型肥胖的药效机制可能与肠道中大肠杆菌、乳酸菌数量的降低及双歧杆菌的数量增加有关。
Objective： To investigate the efficacy mechanisme of chicory extract interventing abdominal obesity rat from the aspect of gut bacteria. Method： Male SD rats were randomly divided into five groups, namely the normal group, model group, large and small dose group of chicory and the fenofibrate group. Normal group was given deionized water, the other group was given fructose water and give the medical treatment of chicory and fenofibrate. Assay triglycerides, total cholesterol, LDL and HDL by biochemical methods and measure body weight and abdominal circumference and microscopicly observe the count changes of gut bacteria through real-time PCR method. Result： Compared with normal group, the triglyceride level and abdominal circumference were significantly higher（P〈0.05）,weight and high-density lipoprotein increased but no significant changes and E. coli, lactobacillus increased significantly. Compared with model group, chicory extract large and small dose group and the fenofibrate group can significantly reduce triglyceride levels（P〈0.05）, reduce the number of E. coli and Lactobacillus and increase the number of bifidobacteria. The fenofibrate group can significantly reduce total cholesterol and high-density lipoprotein levels. Conclusion： The chicory＇s treatment effect on abdominal obesity is significant. The efficacy mechanisme intervention abdominal obesity may be related to the reduction of the number of lactic acid bacteria and E. coli and the increase of bifidobacteria.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第11期2081-2085,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81073068
81274153)
教育部博士点基金项目(20120013130002)
北京中医药大学科研创新团队项目(2011-CD7D-14)
