摘要
目的探讨提取文殊兰Crinum asiaticum种子中总生物碱的最佳提取方法以及工艺条件。方法以文殊兰种子为原料,采用超声和加热回流2种提取方法提取文殊兰种子中的总生物碱。结果响应曲面法优化结果表明,超声波法超声时间47 min,超声温度58℃,超声功率600 W,乙醇体积分数为95%,料液比1∶6,提取3次,提取总生物碱得率0.435 7%;加热回流法提取时间2.5 h,提取温度84℃,料液比1∶9,乙醇体积分数为95%,提取3次,提取总生物碱得率0.449 4%。结论综合考虑总生物碱提取率、操作方法、除杂难易程度等因素,选用加热回流提取文殊兰种子中总生物碱较理想。
Objective To optimize the extracting methods and technology for total alkaloids from the seeds of Crinum asiaticum. Methods Ultrasonic extraction and reflux extraction were used to extract the total alkaloids from the seeds of C. asiaticum. Results The two results showed that the extracting rate of alkaloids by ultrasonic extraction was 0.435 7% under ultrasonic temperature of 58 ℃, ultrasonic time of 47 min, ultrasonic power of 600 W, percentage of ethanol volume of 95%, solid-liquid ratio of 1 ： 6, and extraction for three times. Additionally, the extracting yield of alkaloids by reflux extraction was 0.449 4% under extraction temperature of 84 ℃, extraction time of 2.5 h, solid-liquid ratio of 1 ： 9, percentage of ethanol volume of 95%, and extraction for three times. Conclusion Considering the extracting rate of alkaloids, operating methods, and the difficulty of removing impurity, the heating reflux extraction is an ideal method for total alkaloids extracting from the seeds of Crinum asiaticum.
出处
《中草药》
CAS
CSCD
北大核心
2014年第8期1089-1095,共7页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81274067)
黑龙江省自然科学基金资助项目(D201126)
黑龙江省教育厅科技项目(1211135)
教育部科学技术研究重点项目(211045)
教育部博士点基金项目(20112332110003)
黑龙江省教育厅科技重点项目(12511z011)
哈尔滨市青年科技创新人才专项资金(2013RFQXJ150)
关键词
文殊兰
总生物碱
超声波提取
加热回流提取
响应曲面法
Crinum asiaticum L.
total alkaloids
ultrasonic extraction
heating reflux extraction
response surface methodology
