摘要
以长白楤木为试材,采用超声提取长白楤木的根、茎、叶、芽中皂苷类物质,利用紫外-可见分光光度计测定皂苷类物质的含量,通过单因素试验和正交实验考察了提取时间、料液比、乙醇浓度对皂苷类物质提取率的影响,确定了最佳提取条件。结果表明:料液比对皂苷类物质的提取有显著性影响;料液比为1∶20g/mL、乙醇浓度65%、提取时间20min,为长白楤木中皂苷类成分超声提取的最佳条件,此条件下测得长白楤木中皂苷类物质含量最高的为根,含量为16 461.3465μg/mL。
Taking Aralia continentalis Kitag. as material, saponin of root, stem, leaf and bud were extracted by using ultrasonic method,the content of saponins was measured by ultraviolet-Visible Spectrophotometry,and the factors which influence the saponins extraction rate such as extraction time,the ratio of solid to liquid and concentration of ethanol were explored. The conditions of extraction technology of saponins were optimized by single factor experiment and orthogonal tests to confirm the major factors. The results showed that the extraction rate was affected by the ratio of solid to liquid predominantly,and the optimal conditions extraction technology about ultrasonic extraction as follows.the ratio of solid to liquid was 1 ： 20 g/mL,the concentration of ethanol was 65% and the extraction time was 20 rain. The saponins content of Aralia continentalis Kitag. root was the highest to 16 461. 3465μg/mL.
出处
《北方园艺》
CAS
北大核心
2014年第11期150-153,共4页
Northern Horticulture
基金
吉林农业科技学院校内青年基金资助项目(2012133)
关键词
长白楤木
皂苷
超声提取
紫外分光光度法
最佳工艺
Aralia continentalis
saponin
ultrasonic extraction
Ultraviolet-visible spectrophotom
optimum technology
