摘要
目的 探讨精子畸形与精子染色体异常的关系 ,了解畸形精子的病理学改变。方法 应用光学和电子显微镜、性染色体特异性探针荧光原位杂交 (FISH)技术研究罕见的大头多尾畸形精子。结果 巴氏染色后观察的精子畸形率达 98.75 % (油镜下测量头部畸形达 10 0 % ) ,精子多尾率达 6 0 .2 5 % (最多达 8尾 ) ;电镜观察证实 ,精子头部表面凹凸不平、核型极不规则、有大量细胞质结构 ,尾部除数量异常外 ,尚有中心粒、线粒体和鞭毛结构的异常 ;FISH结果证实 ,性染色体多体率为 6 1.4% ,与精子多尾的组成比有大致的平行关系。结论 尽管体细胞染色体正常 。
Objective To investigate the relationship between sperm anomalies and chromosomal aberrance and to further understand the pathological changes of anomalous spermatozoa.Methods Light and electronic (SEM or TEM) microscopes were used for morphological analysis. Fluorescence in situ hybridization(FISH) with chromosome X ,Y specific probes were used for aneuploidy detection in sperms of the patient. Results Ninety eight point seven five per cent of the Papanicolaou stained sperms were observed to be abnormal; 100% of the sperm heads were abnormal, measured under oil objective (1000×); 60.25% of the sperms had two or more tails (the maximum being eight tails). SEM and TEM demonstrated that the heads contained a large amount of cytoplasmic structure, the surfaces of heads were uneven, the nuclei were of much irregular shape; besides aberrance in quantity, anomalies occurred with centromeres, mitochondria and flagella in the tails. The results of FISH showed that the aneuploidy rate of sex chromosomes was 61.41% which was parallel to the rate of polytails.Conclusion Even though the chromosomes in somatic cells are normal, the chromosomes in anomalous sperms may be abnormal.
出处
《中华医学遗传学杂志》
CAS
CSCD
2001年第1期28-31,共4页
Chinese Journal of Medical Genetics
基金
卫生部科研基金(98-1-109)
