摘要
目的 :探讨抗纤方对TGF β1诱导肝细胞凋亡的抑制作用。方法 :采用细胞生物学等方法 ,利用TGF β1可诱导肝细胞凋亡的特性 ,建立体外实验模型 ,观察抗纤方对TGF β1诱导肝细胞凋亡抑制作用。结果 :实验表明TGF β1以剂量依赖关系诱导肝细胞凋亡 ,当TGF β1浓度在 50 0 pg/ml时可诱导2 2 15细胞凋亡达 63% ,而HepG2细胞凋亡只有 4 2 % ;此时 ,用抗纤方 2 0mg/ml可使 2 2 15细胞凋亡率降至 33% ,HepG2细胞凋亡率降至 2 4 % ,抑制率均在 50 %左右。结论 :抗纤方体外对TGF
To study the inhibitory effect of Kangxian Recipe (KXR) on TGF-β 1 induced hepatocyte apoptosis. Methods: The in vitro model of hepatocyte apoptosis was established by cell biologic methods, utilizing the characteristics of TGF-β 1 to observe the inhibitory effect of KXR on hepatocyte apoptosis. Results: TGF-β 1 induced apoptosis of hepatocyte in a dose-dependent manner. The apoptosis rate of 2.2.15 cells was 63% when 500 ng/L TGF-β 1 was used, while for HepG2 cell, it was merely 44%. After treatment of 20 ng/L KXR, the apoptosis rate of the two kinds of cell lines lowered to 33% and 24% respectively. The inhibiton rate of both groups was about 50%. Conclusion: KXR had strong inhibitory effect on hepatocyte apoptosis induced by TGF-β 1.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2001年第1期37-39,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金重点项目资助课题!(No .39730 4 2 0 )
