摘要
目的：建立高效液相色谱法测定血浆中米非司酮的浓度.方法：采用固相萃取技术处理血浆样品,以炔诺酮为内标,Phenomenex Synergi 4u Fusion-RP 80A C18柱（4.6mm×250mm,4μm）为分析柱,流动相为甲醇-水（75∶25）,流速1.0mL·min-1,柱温：25℃.采用双波长法检测,炔诺酮的检测波长为240nm,米非司酮检测波长为302nm.结果：米非司酮的线性范围为0.01～2.0μg·mL-1,最低定量限为0.01μg·mL-1,回归方程为Y=1.6439X-0.0297,r=0.9998.结论：该方法简便、准确、灵敏,适用于米非司酮药代动力学研究.
Objective：A convenient HPLC method was developd to determine the concentration of mifepristone in plasma. Meth- ods： Solid-phase extration cartridges were used to extract plasma samples . Separation was carried out on a Phenomenex Synergi 4u Fusion-RP 80A C18 （4.6mm× 250mm,4μm）column maintained at 250C with methanol-water（75 ： 25） as mobile phase at a flow rate of 1.0mL· minI. Norethisterone was employed as the internal standard. Dual wavelength was used, with norethisterone monitored at 240 nm and mifepristone at 302 nm. Results：The linearity range of mifepristone was 0.01 ~2.0μg · mL-1 and the lowest limit of quantification was 0.01μg · mL-1 , the regressive equation was Y = 1. 6439X-0. 0297, r= 0. 9998. Conclusion： The method was simple, accurate and sensitive. And it could be applied to study on the pharmacokinetics of mifepristone.
出处
《亚太传统医药》
2014年第11期19-22,共4页
Asia-Pacific Traditional Medicine
关键词
米非司酮
血药浓度
固相萃取
内标法
高效液相色谱法
Mifepristone
Plasma Drug Concentration
Solid-Phase Extraction, Internal Standard
HPLC
