摘要
通过反转录 PCR( RT- PCR)测定了肝窦内皮细胞和枯否细胞中 bcl- 2和 GAPDH m RNA的半衰期。结果表明 ,在窦内皮细胞中 bcl- 2和 GAPDH m RNA的半衰期分别为 6 .13和大于 2 0 h,在枯否细胞中 bcl- 2和GAPDH m RNA的半衰期分别为 5 .86和 13.74h。
In the study,half lives of bcl 2 and GAPDH mRNA were determined in isolated sinusoidal endothelial cells (SEC) and Kupffer cells (KC) of rat livers during Thioacetamide induced Fibrosis by RT PCR.The results showed that half lives of bcl 2 and GAPDH mRNA were 6.13 hours and over 20 hours respectively in sinusoidal endothelial cells,and 5.86 hours and 13.74 hours respectively in Kupffer cells.
出处
《西北农业大学学报》
CSCD
北大核心
2001年第1期15-18,共4页
Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition)
基金
德国 DFG基金资助项目