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肝窦内皮细胞和枯否细胞中bcl-2和GAPDH mRNA半衰期的测定

Half life determination of bcl-2 and GAPDH mRNA in sinusoidal endothelial cells and Kupffer cells of rat liver
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摘要 通过反转录 PCR( RT- PCR)测定了肝窦内皮细胞和枯否细胞中 bcl- 2和 GAPDH m RNA的半衰期。结果表明 ,在窦内皮细胞中 bcl- 2和 GAPDH m RNA的半衰期分别为 6 .13和大于 2 0 h,在枯否细胞中 bcl- 2和GAPDH m RNA的半衰期分别为 5 .86和 13.74h。 In the study,half lives of bcl 2 and GAPDH mRNA were determined in isolated sinusoidal endothelial cells (SEC) and Kupffer cells (KC) of rat livers during Thioacetamide induced Fibrosis by RT PCR.The results showed that half lives of bcl 2 and GAPDH mRNA were 6.13 hours and over 20 hours respectively in sinusoidal endothelial cells,and 5.86 hours and 13.74 hours respectively in Kupffer cells.
出处 《西北农业大学学报》 CSCD 北大核心 2001年第1期15-18,共4页 Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition)
基金 德国 DFG基金资助项目
关键词 RT-PCR 肝窦内皮细胞 枯否细胞 mRNA半衰期 BCL-2 GAPDH 基因表达 RT PCR sinusoidal endothelial cells Kupffer cells mRNA half life determination
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