摘要
考察谷氨酸棒状杆菌ATCC13032Δldh厌氧产丁二酸的发酵条件。结果发现:补加NaHCO3的效果最好,并且考察了NaHCO3浓度对葡萄糖转化速率及丁二酸生成速率的影响。运用代谢流分析方法分析了乳酸脱氢酶基因敲除对谷氨酸棒状杆菌厌氧代谢的影响,发现乳酸脱氢酶基因敲除导致磷酸烯醇式丙酮酸生成丁二酸的流量提高了214.3%,流向乳酸的流量变为0;分批厌氧转化36 h生成41.2 g/L丁二酸,产率45.0%。
The conversion conditions of Corynebacterium glutamicum ATCC13032Δldh under anaerobic condition were investigated.The optimal carbonates were bicarbonate,and the rate of sugar consumption and succinic acid production were influenced by bicarbonate concentration.The effects of lactate dehydrogenase deletion in Corynebacterium glutamicum under anaerobic metabolism were investigated by the metabolic flux analysis and the metabolic flux to the succinic acid synthesis pathway increased by 214.3%, while the metabolic flux to lactic acid synthesis pathway became zero.Succinic acid concentration reached 41.2 g/L within 36 h and the yield of succinic acid was 45.0%.
出处
《生物加工过程》
CAS
CSCD
2014年第3期7-11,共5页
Chinese Journal of Bioprocess Engineering
基金
国家自然科学基金(20576055)
关键词
厌氧转化
谷氨酸棒状杆菌
乳酸脱氢酶基因
代谢流
丁二酸
anaerobic transformation
Corynebacterium glutamicum
lactate dehydrogenase gene
metabolic flux
succinic acid
