摘要
为了探索锥形纳米孔整流的机理，以硼硅酸盐玻璃毛细管为材料，采用微电极拉制仪P 2000拉制了内径20～50 nm的玻璃锥形纳米孔。孔内注满氯化钠盐溶液后，采用膜片钳对该类纳米孔的整流效应进行了实验研究。实验发现，锥形玻璃纳米孔的整流比随浓度先增大后减小，在溶液浓度为10～50 mmol/L范围内出现最大整流比。当溶液pH值高于玻璃等电点时，随着溶液pH值的增大，整流比随之增大；当溶液pH值低于玻璃等电点时，玻璃表面发生壁面电荷倒置，整流方向发生改变。实验结果表明，玻璃锥形纳米孔整流效应受到溶液浓度和溶液pH值共同影响，纳米孔内壁不经过任何化学修饰就可以发生电荷倒置。
In order to explore the mechanism of the conical nanopore rectification,glass conical nan-opores were fabricated with boron silicate glass capillary on micropipette puller system P 2000.The nanopores＆#39; diameter ranged from 20 to 50 nm.After the pore was filled with sodium chloride solu-tion,the rectification effect of the pore was experimentally studied with a patch clamp.It is found that the rectification ratio of the nanopore shows an increase and then decrease trend as the solution concentration becomes higher,and the highest rectification ratio appears when the salt concentration is between 10 and 50 mmol/L.When the pH is above the isoelectric point of glass,the rectification ratio increases as the solution pH increases.When the pH is below the isoelectric point of glass,the surface of the glass exhibits charge inversion and the rectification direction changes.The results show that conical nanopore rectification can be influenced both by salt concentration and pH value,and charge inversion can occur on the surface of glass without any chemical modification.
出处
《东南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2014年第3期526-530,共5页
Journal of Southeast University：Natural Science Edition
基金
国家重点基础研究发展计划(973计划)资助项目(2011CB707601
2011CB707605)
关键词
浓度
PH值
整流
玻璃纳米孔
concentration
pH value
rectification
glass nanopore
