摘要
目的:观察紫杉醇对裸鼠体内涎腺腺样囊性癌移植瘤的抑制作用。方法:按常规将涎样囊性癌ACC-M细胞接种于裸鼠皮下,2周后将荷瘤鼠随机分为2组(n=8),对治疗组鼠皮下注射紫杉醇,10μg/g,1次/d。对照组鼠给予等量生理盐水。定期测量肿瘤体积,治疗2周后处死,测量肿瘤重量,病理学观察,流式细胞术检测各组凋亡率。免疫组化法检测血管内皮生长因子(VEGF)在肿瘤细胞中的表达。结果:治疗2周后紫杉醇治疗组抑瘤率42.74%,治疗组和对照组肿瘤细胞凋亡率分别为(24.3±2.1)%和(9.2±1.9)%;VEGF蛋白表达平均吸光度值(MOD)分别为0.322±0.033和0.388±0.029(P<0.01)。结论:紫杉醇能够抑制ACC-M细胞在裸鼠体内的增殖,促进其凋亡,抑制VEGF的表达。
Objective： To investigate the effects of paclitaxel（PTX） on the proliferation of and VEGF expression adenoid cystic carcinoma ACC-M cells in nude mice. Methods： ACC-M cells were injected subcutaneously into 16 BALB/c nude. The mice with tumor were randomly divided into 2 groups（n =8）. The mice in PTX group were given PTX at 10 μg/g, 1/d. The mice in control group were given normal saline. Tumor growth was measured regularly. Treatments were conducted for 2 weeks, then the mice were sacrificed, tumor weight was measured, apoptosis and VEGF expression of the tumor cells were examined by flowcytometry and immunohistochemical staining, respectively. Results： After 2-week-treatment the tumor in PTX group was inhibited by 42.74%. The apoptosis rates in PTX and control groups were （24.3 ± 2.1 ）% and （9.2 ± 1.9 ）%, respectively. Mean optical density （MOD） of VEGF pro- tein in PTX and control groups was 0. 322 ± 0.033 and 0.388 ± 0. 029 respectively（ P 〈 0.01 ）. Conclusion： Paclitaxel can inhibit the proliferation, down-regulate the expression of VEGF and promote apoptosis of ACC-M cells in nude mice.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2014年第3期327-330,共4页
Journal of Practical Stomatology
基金
云南省科技厅-昆明医科大学联合专项基金(编号:2011FB211
2011FB163)
云南省高层次卫生技术人才专项基金(编号:D-201233)
