摘要
为获知大黄鱼染色体的物理长度,实验采用流式细胞术测定了大黄鱼基因组大小,并利用显微图像分析技术测定了大黄鱼24对染色体的面积和累积光密度(IOD),据此估算了大黄鱼染色体的物理长度。结果显示,大黄鱼基因组大小约为(725.25±14.65)Mb;染色体相对面积最小为2.26%±0.08%,最大为4.83%±0.08%;染色体相对IOD最小为1.80%±0.32%,最大为5.04%±0.15%;物理长度最小的是24号染色体,为(13.1±3.37)Mb,其他染色体的物理长度为(24.4±6.21)^(36.6±1.62)Mb。染色体的物理长度与相对面积、相对长度分别成正线性相关。其中,物理长度-相对面积的相关系数大于物理长度-相对长度。显微图像还显示,同一基因组中的染色体碘化丙啶(PI)着色不完全均一,非同源染色体间、部分同源染色体间以及染色体不同位置均存在荧光强度的差异。研究结果为大黄鱼染色体识别与配对提供了新的数量标记,也为深入解析大黄鱼基因组结构提供基础数据。
The sizes of genome and chromosomes were respectively estimated with flowcytometry and image analysis in large yellowcroaker（ Larimichthys crocea）. Genome size of large yellowcroaker was estimated at 725. 25 ± 14. 65 Mb. The relative area of chromosome ranged from 2. 26% ± 0. 08% to 4. 83% ± 0. 08%; the relative integrated optical density（ IOD） ranged from 1. 80% ± 0. 32% to 5. 04% ± 0. 15%. Based on the relative IOD,the shortest physical length was estimated at 13. 1 ± 3. 37 Mb for the 24th chromosome,while the physical length of the other chromosomes ranged from 24. 4 ± 6. 21 Mb to 36. 6 ± 1. 62 Mb. Linear correlation between the physical length and the relative area was observed,as well as linear correlation between the physical length and the relative length. The correlation coefficient for the physical length and the relative area was higher than that for the physical length and the relative length. The image of metaphase chromosome also showed that the optical density of chromosomes was not completely uniform with propidium iodide（ PI） staining. The variation existed among non-homologous chromosomes,between sister chromatids,and among different regions of a chromosome. Above all,newparameters of chromosomes present in this paper will facilitate chromosome identification and pairing,as well as studies on genomics,in large yellowcroaker.
出处
《水产学报》
CAS
CSCD
北大核心
2014年第5期632-637,共6页
Journal of Fisheries of China
基金
国家自然科学基金(31272653)
国家"八六三"高技术研究发展计划(2012AA10A403)
福建省自然科学基金(2013J01135
2011J01228)
关键词
大黄鱼
染色体
基因组大小
染色体的物理长度
图像分析
Larimichthys crocea
chromosome
genome size
physical length of chromosome
image analysis
