摘要
目的:研究β淀粉样肽(Aβ)处理SH-SY5Y细胞以及α3神经型尼古丁受体(α3 nAChR)沉默细胞后细胞Notch信号通路的变化。方法:2μmol/L Aβ处理SH-SY5Y细胞以及α3 nAChR沉默细胞,用实时荧光定量聚合酶链式反应(Real Time PCR)和蛋白印迹(western blot)方法分别检测细胞中Notch信号通路中Notch1和Hes1的mRNA及蛋白水平的变化,评价Aβ对细胞Notch信号通路的影响。结果:Aβ处理的SH-SY5Y细胞以及α3 nAChR沉默细胞中Notch1和Hes1的mRNA及蛋白水平表达均增加,且在α3 nAChR沉默细胞中增加更为明显,差异有统计学意义(P<0.05)。结论:Aβ可增加细胞Notch信号通路基因表达,α3 nAChR沉默会增强Aβ对Notch信号通路基因表达的影响,这可能与阿尔茨海默氏病(AD)的发病有一定的关系。
Objective： To observe the changes of Notch signal transduction pathway in SH-SY5Y neuroblastoma cells and 3 nAChR silence ceils after treated with β-amyloid peptide （A β）. Methods： SH-SYSY cells and 3 nAChR silence cells were exposed to 2 μmol/L A β1-42. The mRNA and protein levels of Hesl and Notchl of Notch signal transduction pathway were measured by using Real-Time PCR and Western Blotting methods respectively. Results： mRNA and protein levels of Notchl and Hesl increased both in A β treated SH-SY5Y cells and 3 nAChR silence cells, and specially in 3 nAChR silence ceils, the increase was more obvious. The differences were statistically significant （P 〈 0.05 ）. Conclusions： A β may enhance the gene expression in Notch signal transduction pathway; and the silence in α3 nAChR cells can enhance such effects, which might be connected to the patho- genesis of AD.
出处
《贵阳医学院学报》
CAS
2014年第2期154-157,161,共5页
Journal of Guiyang Medical College
基金
国家自然科学基金资助项目(81360178)
贵州省科技厅社会发展攻关项目(黔科合SY字[2013]3020号)
优秀青年人才项目(黔科合人字(2013)44号)
贵州省教育厅优秀科技人才项目(黔教合KY字[2012]089号)
