摘要
[目的]研究新型靶向光敏剂Ⅰ光动力诱导HeLa细胞死亡的方式。[方法]用流式细胞仪分析照射剂量和光敏剂浓度对HeLa细胞凋亡的影响,细胞凋亡和坏死的比例;并采用激光共聚焦观察细胞F-actin和核的变化;透射电镜观察细胞超微结构的改变。[结果]光敏剂Ⅰ介导的光动力治疗可诱导HeLa细胞的凋亡和坏死,但凋亡的比例明显大于坏死(P=0.0053),且细胞凋亡的量分别与光照剂量(r=0.987,P<0.001)和光敏剂浓度(r=0.995,P<0.001)呈正相关;光动力诱导后,细胞的F-actin结构破坏,细胞皱缩,透射电镜下可见凋亡小体的形成。[结论]光敏剂Ⅰ介导的光动力治疗主要通过凋亡途径导致HeLa细胞的死亡。
[Purposel To study the death mode of HeLa cells induced by a new targeting photo- sensitizer I -based photodynamic therapy （PDI）. [Methods] The influence of irradiation dose and the concentration of photosensitizer I on the apoptosis of HeLa cells,and the percentage of apoptosis and necrosis of HeLa cells were analyzed by flow cytometry. The changes of F- actin and nuclear of HeLa cells were observed through confoca]. The changes of ultrastructure were observed under transmission electron microscope. [Results ] Compared with control cells, photosensitizer I -based PDT could induce apoptosis and necrosis in HeLa cells,but the percentage of apoptotic cells was obviously more than that of necrosis, and the number of apoptotic cells displayed positive correlation with the irradiation dose and the concentration of photosensitizer I ,the correlation coefficients were 0.987 （P〈0.O01） and 0.995 （P〈0.001） respectively. After PDT,the F-actin of HeLa cells was damaged, the nucleus shrank, and the apoptotic bodies could be observed under transmission electron microscope. [Conclusion] Apoptosis was the main death mode of HeLa cells induced by photosensitizer I -based PDT.
出处
《肿瘤学杂志》
CAS
2014年第5期383-387,共5页
Journal of Chinese Oncology
基金
国家自然科学基金资助项目(21072227)
