摘要
目的：观察不同年龄阶段健康人群曝光皮肤组织中紧密连接蛋白claudin1分子的表达情况，探讨其与表皮屏障功能之间的关联性，并分析慢性紫外线照射对皮肤组织中claudin1蛋白的表达及表皮屏障功能的影响。方法：收集不同年龄共39人曝光部位皮肤组织（包括颈项部、手背部或额面部），其中〈20岁组9例，20～50岁组14例，〉50岁组16例。分别以RT—PCR检测皮肤组织中的claudin1分子转录情况，以免疫组化法检测claudin1蛋白的表达，并检测不同皮肤组织的经皮水分丢失（TEWL）值。结果：RT—PCR检测显示cluadin 1分子的转录水平均随年龄增大而下调，〉50岁组claudin1分子的mRNA表达较〈20岁组明显降低（P=0．0370）。免疫组化结果显示曝光部位皮肤组织中claudin1蛋白的表达强度随年龄增长而减弱，与年龄呈负相关，与RT—PCR结果一致。老年组（〉50岁组）曝光部位皮肤的TEWL值较中年组（20—50岁组）及青年组（〈20岁组）均升高，TEWL值与claudin1蛋白的表达呈负相关（P=0．0475）。结论：慢性紫外线照射可引发人曝光部位皮肤的屏障功能损伤，而这种屏障功能受损可能与claudin1蛋白的下调表达有关。
Objective： To analyze the effect of chronic UV exposure on expression of tight junction protein and epidermal barrier function via measuring the expression levels of claudinl and barrier function in UV-exposed skin of different ages, and to determine the association between claudinl expression and epidermal barrier function. Methods： UV-exposed skin samples were obtained from the neck, back of hands, forehead or face of 39 volunteers, including 9 young （〈20 y group）, 14 middle- aged （20-50 y group） and 16 old （〉50 y group）. RT-PCR was performed to detect the mRNA levels of claudinl, and immunohistochemistry was used to analyze the expression of claudinl protein. Transepidermal water loss （TWEL） was also measured. Results： RT-PCR showed that the levels of claudinl mRNA in UV-exposed skin were decreased with age, particularly in old group （P=0.0370, 〉50 y group vs. 〈20 y group）. Immunohistochemistry revealed that expression levels of claudinl protein were negatively correlated with age. TEWL readings in old group （〉50 y group） were higher than that in middle-aged group （20-50y group） and young group （〈20 y group）. A negative correlation between TEWL and claudin 1 was observed（P=0.0475）. Conclusion： Abnormal epidermal permeability barrier function can be induced by chronic UV exposure, possibly due to the down-regulation of claudinl protein.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2014年第6期340-343,共4页
Journal of Clinical Dermatology
基金
2010年中华医学会-欧莱雅中国人健康皮肤研究基金(S2011080804)
浙江省卫生厅科研基金(2009B045)资助项目
