摘要
目的观察FTY720对创伤性休克兔急性肺损伤的保护作用。方法采用Lamson′s法建立创伤性休克动物模型。取72只家兔随机分为6组,即假休克组(CON)、乳酸钠林格氏液(LR)复苏组、乳酸钠林格氏液联合地塞米松(LR/DXM)复苏组、乳酸钠林格氏液联合FTY720(LR/FTY720)复苏组,其中FTY720分为低剂量(FTY720-L)、中剂量(FTY20-M)、高剂量(FTY20-H),剂量分别为0.1mg/kg、0.3mg/kg、0.9mg/kg。复苏过程中监测血流动力学变化,复苏后2h测血气分析。采用ELISA法检测血浆中及支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中TNF-α、IL-6、IL-10的变化。用BCA法检测BALF中蛋白含量。复苏后2h各组随机处死6只动物,用HE染色观察肺组织的病理变化。各组剩余动物(每组6只)给予正常饮食观察24h、48h、72h的存活率。结果与LR组比较,各组间差异有统计学意义(P<0.05);与DXM组相比,各组间差异有统计学意义(P<0.05);FYT720组内比较差异有统计学意义(P<0.05),即应用FYT720-M复苏组肺组织的病理损害明显减轻,血浆及BALF中TNF-α、IL-6含量明显降低,IL-10含量明显提高,72h存活率明显提高。结论FTY720对创伤性休克兔所致急性肺损伤有保护作用,并且提高了创伤后的存活率。
Objective To observe the protective effect of FTY720 on acute lung injury（ALI） in a rabbit model of traumatic shock. Methods The traumatic shock model was established from improved Lamson＇s method. 72 rabbits were randomly divided into 6 groups（n=12 each）：group CON,group LR,group LR/DXM,group LR/FTY720（0.1mg/kg,0.3mg/kg,0.9mg/kg）. Observed the change of hemodynamic in the process of fluid resuscitation. 2 hours after resuscitation, the blood gas analysis was measured. Enzyme immunosorbent test was used to detect the IL-6,IL-10,TNF-α level in blood and bronchoalveolar lavage fluid（BALF）. Measured the total protein in BALF by BCA method. 2 hours after resuscitation, the 6 rabbits were randomly killed and observed the pathology change of lung organization by HE dye. The left rabbits（n=6,each group） fed normal food was observed for 24h,48h,72h to determine survival rate. Results Compared with other groups the damage of lung organization was lessened significantly and the expression of IL-6,IL-10,TNF-α decreased in FTY720-M group. But the IL-10 and survival rate increased in FTY720-M group. Conclusion FTY720 has protective effect on ALI for Traumatic shock rabbit, and it can increase the survival rate after trauma.
出处
《中国现代医药杂志》
2014年第4期27-31,共5页
Modern Medicine Journal of China
