摘要
以3个大豆品种为试材，研究了种子消毒方法、丛生芽诱导和生根阶段不同的激素浓度对大豆子叶节再生的影响，以求建立一个高效的再生体系用于大豆的遗传转化。结果表明，大豆种子消毒的最佳方法为：70％酒精消毒30 s后，HgC12消毒7 min，无菌水清洗3-5次。吉农28的最佳丛生芽诱导培养基为MS＋1．5 mg/L 6-BA＋0．5 mg/L IBA；而对于吉农27和九农21，MS＋2．0 mg/L 6-BA＋0．8 mg/L IBA为最佳丛生芽培养基。在丛生芽诱导生根阶段，三种不同的基因型最佳的生根培养基均为1/2MS＋2．0 mg/L IBA。
Using three soybean varieties for materials,the effects of seed disinfection method,different hormone concentrations at bud induction and rooting stages on the regeneration of soybean cotyledon node were studied to establish an efficient regeneration system for soybean genetic transformation.The results showed that the best seed disinfection method was disinfecting with 70%alcohol for 30 seconds,then HgCl2 for 7 minutes, and finally washing 3-5 times with sterile water.The optimum bud induction medium for Jinong 28 was MS＋1.5 mg/L 6-BA ＋0.5 mg/L IBA;for Jinong 27 and Jiunong 21,MS＋2.0 mg/L 6-BA＋0.8 mg/L IBA was the best.During the rooting stage,the best rooting mediums for three soybean varieties were all 1/2MS ＋2.0 mg/L IBA.
出处
《山东农业科学》
2014年第5期26-28,共3页
Shandong Agricultural Sciences
基金
吉林农业大学科研启动基金(201242)
吉林省科技厅科技发展计划项目(201101111)
吉林省教育厅科学技术研究资助项目(2011-41)
吉林省科技厅项目(20120215)
吉林省科技厅成果转化补助项目(20095044)
关键词
大豆
激素
丛生芽诱导
生根
Soybean
Hormone
Bud induction
Rooting
