摘要
目的:构建靶向COX-2基因的有效shRNA干扰载体并进行鉴定分析。方法:根据Genbank提供的COX-2 mRNA序列,使用Designer3.0(Genepharma)软件进行设计DNA oligo,构建并化学合成shRNA模板,用酶切和测序方法进行分析鉴定。结果:通过酶切和序列测定表明,成功构建了COX-2 shRNA表达载体。结论:构建的COX-2 shRNA干扰载体,为下一步转基因研究,探讨COX-2功能奠定基础。
Objective：To construct effective COX -2 gene targeting shRNA interference vector .Methods：design and synthesize shR-NA template, using DNA oligo （Genepharma） software Designer3.0 according to Genbank COX -2 mRNA sequence.Results：The plas-mid was confirmed to be constructed as expectation by enzyme digestion and sequence reaction .Conclusion：COX-2 shRNA interference vectors could be constructed successfully , should lay a foundation for the follow -on genetically modified experiments and exploring COX-2 function .
出处
《中国伤残医学》
2014年第10期39-41,共3页
Chinese Journal of Trauma and Disability Medicine
基金
国家自然科学基金资助项目(81171774
81272056)
