摘要
目的:探讨β-catenin在卵巢子宫内膜异位症在位及异位子宫内膜间质细胞的表达及意义。方法:体外分离、纯化及培养OEms患者在位、异位和对照组子宫内膜间质细胞,应用逆转录聚合酶链反应(RT-PCR)方法检测上述各组细胞中β-catenin的表达水平。结果:分离得到的内膜间质细胞纯度达95%以上。卵巢子宫内膜异位症在位内膜、异位内膜和对照组中β-catenin的IOD值分别为(0.672±0.07)、(0.351±0.029)和(0.184±0.020),各组间均有统计学差异(F=27.68,P<0.01)。结论:Wnt/μ-catenin信号通路可能在卵巢子宫内膜异位症发生、发展过程中发挥重要作用。
Objective： To explore the expression and significance of β-catenin in interstitial cells of eutopic endometrium and ectopic endometrium of ovarian endometriosis. Methods： The interstitial cells in eutopic endometrium and ectopic endometrium of patients with ovarian endometriosis and normal endometrium of cases in control group were isolated in vitro,purified and cultured,RT- PCR was used to detect the expression levels of β- catenin in interstitial cells of the above- mentioned groups. Results： The purify of interstitial cells was more than 95%. IOD values of β- catenin in eutopic endometrium and ectopic endometrium of ovarian endometriosis group and normal endometrium of control group were（ 0. 672 ± 0. 07）,（ 0. 351 ± 0. 029） and（ 0. 184 ± 0. 020）,respectively,there was statistically significant difference（ F = 27. 68,P〈 0. 01）. Conclusion： Wnt /β- catenin signaling pathway may play an important role in pathogenesis and development of ovarian endometriosis.
出处
《中国妇幼保健》
CAS
北大核心
2014年第16期2495-2497,共3页
Maternal and Child Health Care of China
基金
江西省自然科学基金〔2012BAB205011〕
