摘要
目的研究降糖中成药中非法添加格列类药品的表面增强拉曼光谱(surface enhanced Raman spectroscopy,SERS)检测方法。方法利用薄层色谱法将待检成分与中药基质进行简单分离,采用表面增强拉曼光谱技术对薄层板上的微量物质进行检测。通过摸索模拟阳性样品中格列类药品的SERS检测条件,建立可用于降糖中成药中非法添加物的检测方法。结果采用有机溶剂DMF制备所得的银溶胶可以获得较好的格列类药物SERS图谱。结论该研究所建立的TLC与SERS联用方法检测简便、快速、经济,可用于降糖中成药中非法添加格列类药品的快速检测。
Objective To establish detection method of glinides adulterated illegally in hypoglycemic traditional Chinese medi -cine （TCM） by surface enhanced Raman spectroscopy （SERS）.Methods Adulterated chemicals and TCM matrixes were separated by TLC first.Then trace substances in TLC plate was tested by SERS method .By investigating SERS detection conditions of glinides chemi-cals in simulated positive samples , a detection method was established to detect illegal adulterant in hypoglycemic TCM .Results Better SERS spectra of glinides could be obtained by silver sol prepared with organic solvent DMF .Conclusion The detection method coupled with TLC and SERS in this paper was simple , fast and economical which could be used to detect glinides adulterated illegally in hypogly -cemic Chinese patent medicine quickly .
出处
《药学实践杂志》
CAS
2014年第3期206-208,230,共4页
Journal of Pharmaceutical Practice
关键词
表面增强拉曼光谱
中药制剂
非法添加
格列类药物
surface enhanced Raman spectroscopy
traditional Chinese medicine
adulteration
glinides
