摘要
以光合作用高温稳定性差异明显的籼型水稻品种蜀恢527和特籼占25为材料,研究开花期高温对其功能叶片相关生理特性的影响。结果表明:高温胁迫下光合作用高温稳定型品种蜀恢527的净光合速率及结实率降幅较小,叶片脯氨酸积累量增幅较大,细胞膜伤害率明显较低;同时剑叶Rubisco大亚基、小亚基和Rubisco粗酶液中总蛋白质含量的50.0℃热稳定性均较高。Rubisco的大、小亚基中,以小亚基的稳定性对温度变化反应最为敏感,可为水稻抽穗开花期耐热性资源鉴定、评价及机制研究提供一定的参考。
Effects of high temperature at flowering stage on the related physiological characteristics of functional leaves of rice were studied using indica rice varieties-Shuhui 527and Texianzhan 25as samples,which had significant differences in high temperature stability of photosynthesis.The results showed that under heat stress,Shuhui 527with high temperature tolerance of photosynthesis presented small decreasing ranges of net photosynthetic rate and seed setting rate,large increasing range of proline cumulative level in leaves and significantly lower membrane injury rate.In addition,the thermal stability at 50.0℃of large subunit of Rubisco（Lsu）,small subunit of Rubisco（Ssu）and total protein content in Rubisco-extracting solution of flag leaves was high in Shuhui 527.Between Lsu and Ssu,the stability of Ssu was more sensitive to changes in temperature.The thermal stability of Ssu could provide a reference for studies on the identification,evaluation and mechanism of high temperature tolerance of rice at flowering stage.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2014年第3期7-11,共5页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31371600)
关键词
水稻
开花期
高温胁迫
光合作用高温稳定性
结实率
生理特性
rice
flowering stage
heat stress
high temperature stability of photosynthesis
seed setting rate
physiological characteristics
