摘要
目的证明使用全自动快速分枝杆菌培养仪(BD960)能用于肺内、肺外临床标本(除血液、尿液)快速培养分枝杆菌,除传染科外其他科室执行危急值报告制度确保医疗安全。方法收集临床标本共596份,根据不同的标本,进行标本预处理后,分别接种于BBLTMPrepared Culture Media分枝杆菌培养管,放入全自动快速分枝杆菌培养仪(BD960)培养,并将标本同步进行抗酸染色及接种血平板。经培养,如仪器报警阳性,且培养物抗酸染色阳性,将培养物用荧光聚合酶链反应(PCR)方法进行菌株鉴定,鉴定分为结核分枝杆菌与非结核分枝杆菌。结果 596份临床标本中,共培养出61株分枝杆菌,阳性率为10.2%(61/596),其中结核分枝杆菌占82.0%(50/61);非结核分枝杆菌占18.0%(11/61)。全自动快速分枝杆菌培养仪(BD960)阳性标本检出时间较快,阳性标本最早4d,平均检出时间为10d。结论综合医院使用全自动快速分枝杆菌培养仪(BD960)能快速培养分枝杆菌,做到早诊断、早隔离、早治疗,控制结核病传染,控制综合医院医院感染,确保医疗安全。
Objective To explore the application of fast mycobacterial culture instrument （BD960） for the fast culture of mycobacterial from intra-pulmonary or extra-pulmonary specimens .Methods A total of 596 cases of clini-cal specimens were collected ,pretreated ,inoculated in BBLTM Prepared Culture Media mycobacterium culture tube , then cultured in BD960 instrument .All specimens were synchronously detected for acid-fast staining and inoculation of blood plate .Mycobacterium tuberculosis or non-Mycobacterium tuberculosis in culture was identified by using flu-orescence polymerase chain reaction （PCR） ,if instrument warned for positive alarm and the culture was positive for acid-fast stain .Results In 596 clinical specimens ,61 strains of mycobacteria were obtained ,and the positive rate was 10 .1% （61/596） ,including Mycobacterium tuberculosis for 82% （50/61） and non-Mycobacterium tuberculosis for 18 .0% （11/61） .Positive specimens could for the fast be detected within 4 days by culturing of BD960 ,and the aver-age time need for detection for positive specimens was 10 days .Conclusion Application of BD960 instrument in gen-eral hospital could be helpful for the fast culture of mycobacterium ,for early diagnosis ,isolation and treatment of pa-tients ,also for the control of tuberculosis infection and nosocomial infections .
出处
《检验医学与临床》
CAS
2014年第10期1353-1355,共3页
Laboratory Medicine and Clinic
关键词
结核
全自动快速分枝杆菌培养仪
结核分枝杆菌
非结核分枝杆菌
医院感染
tuberculosis
automatic fast mycobacteria culture system
Mycobacterium tuberculosis
mon-Mycobacterium tuberculosis
nosocomial infection
