摘要
近年来,量子点以其独特的光学性质被广泛应用到医学检测上.血清中人绒毛膜促性腺激素(HCG)的含量是诊断早期妊娠的常用指标,也可用于异常妊娠性疾病的早期发现和鉴别诊断.本文采用超声乳化法制备了高质量的亲水性量子点,并将其与免疫层析试纸条技术相结合,在此基础上自主研发了用于试纸条检测的量子点免疫荧光检测仪,对血清中HCG的含量进行了高灵敏度的快速定量检测.结果表明,对于血清中的HCG含量检测,最优检测条件为加样体积50μl,反应时间15 min,检测的灵敏度达到0.85 U/L,高于商品化的胶体金试纸条.这种检测技术简单、快速、灵敏,有望在其他蛋白质类标志物的检测中得到广泛应用.
In this work, we developed a novel fluorescent biosensor that integrates a lateral flow test strip with quantum dot （QD） and a home-made test strip reader for rapid, low-cost, quantitative, and sensitive human chorionic gonadotropin （HCG） detection. The serum HCG level is a reliable marker for detecting persistent gestational trophoblastic neoplasia （GTN） and reflects the clinical course of the disease. The biosensor is designed to combine the rapidness of lateral flow immunoassay and sensitivity of fluorescent immunoassay. The principle of this biosensor is on the basis of a sandwich immunoreaction that was performed on the test strip and the QDs are captured on the test line through antibody-antigen reaction and on the control line through the immobilized secondary antibody. Quantitative detection is realized by recording the intensity of the test line and control line with our home-made test strip reader. The results are determined by the ratio of the fluorescence intensity of the test line and control line （IT/Ic）, which would cancel out the influence of the stability of QDs and antibody. The immunoreaction time and the sample volume are optimized to get a higher sensitivity. Under optimal conditions, the QD-based lateral flow immunoassay system has a wide dynamic range and is capable of detecting a minimum 0.85 IU/L HCG standard analyte in 15 min with 50μl sample volume. The novel QD-based lateral flow immunoassay biosensor offers a rapid, sensitive and quantitative tool for point-of-care testing of the level of serum HCG and shows great promise in other protein markers.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2014年第5期497-507,共11页
Progress In Biochemistry and Biophysics
基金
国家高技术研究发展计划(863)资助项目(2012AA022603)~~
