摘要
本文报道了水稻第1染色体长臂上微效千粒重QTL qTGW1.2的验证和分解。针对前期qTGW1.2定位结果,应用SSR标记检测,从籼籼交组合珍汕973/密阳46衍生的1个BC2F7分离群体中,筛选到杂合区间分别为RM11621-RM297和RM212-RM265的2个单株,构建了2套BC2F8:9近等基因系,将qTGW1.2进一步界定在RM212-RM265及其两侧交换区间的区域内。在此基础上,筛选出5个在目标区间内分离片段缩小且呈阶梯状排列的单株,衍生了5套BC2F10分离群体,应用Windows QTL Cartographer 2.5进行QTL分析。结果表明,每套群体均检测到千粒重QTL,加性效应为0.13～0.38 g,来自密阳46的等位基因提高千粒重;经比较各个群体的分离区间,将qTGW1.2分解为互引连锁的2个QTL,其中,qTGW1.2a位于RM11730和RM11762之间934 kb的区域内,呈加性作用,qTGW1.2b位于RM11800和RM11885之间2.1 Mb的区域内,呈正向超显性。
Validation and dissection of a minor QTL q TG W1.2 for 1000-grain weight located on the long arm of rice chromosome 1 was reported. Following previous mapping result, two plants carrying heterozygous segments covering the intervals RM11621-RM297 and RM212-RM265, respectively, were selected from the Zhenshan 973/Milyang 46 BC2F7 population. Two sets of near isogenic lines （NILs） in the BC2F8：9 generation were established. QTL analysis using the two NIL sets delimited q TG W1.2 to a region flanked by RM 11730 and RM 11885. Then, five B C2F9 plants with sequential heterozygous segments over- lapped in the region covering qTGW1.2 were selected. From the selfed seeds five BC2F10 populations were constructed and used for QTL analysis by Windows QTL Cartographer 2.5. QTLs for 1000-grain weight were detected in each of the five populations. The additive effect ranged from 0.13 to 0.38 g with the enhancing alleles derived from Milyang 46. Based on comparison of the segregating regions among the five populations, we separated qTGW1.2 into two QTLs, of whieh qTGW1.2a displaying additive genetic action was located in a 934 kb region flanked by RM11730 and RM11762, and qTGW1.2b displaying positive over-dominance was located in a 2.1 Mb region flanked by RM11800 and RM11885.
出处
《作物学报》
CAS
CSCD
北大核心
2014年第5期761-768,共8页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31221004)
国家超级稻育种专项(201301)
水稻生物学国家重点实验室自主研究课题(ZZKT200801)资助
