摘要
以美国红枫为母本,我国原产的元宝枫为父本,采用直接授粉法、切除柱头法、切除柱头加化学试剂法3种方法在温室条件下,用超低温保存的元宝枫花粉对带有花芽的离体美国红枫枝条进行授粉实验;子房发育约20~25d之后,分别采集经不同处理的子房对其进行表面灭菌处理后,接种于1/2MS+6-BA(0.01mg/L)+NAA(0.1mg/L)的培养基上进行胚抢救培养。结果表明:含水量为12.2%的花粉经超低温保存一年之后,保持着与新鲜花粉相当的活力。3种不同的授粉方法中,生长情况最好的为直接授粉法,切除柱头加化学试剂法次之,而切除柱头发效果最差。通过胚抢救技术获得了一定量的杂种后代株系。
In this research, Acer ruhrum was chosen as female parent, while Acer truncatum which is native to China was regarded as the male parent. Direct stigma pollination, cut--stigma pollination,and cut--stigma pollination with chemical reagent were carried out for pollination. Ovaries with different pollination method were collected 20~25d after pollination and sterilized. Embryo rescue cultured on the I/2MS medium supplemented with 4mg/L6--BA and 0.5mg/L IBA. The results showed that pollen with 12.2 ~/＇~0 moisture content maintained the highest pollen germination relative to fresh pollen. Stigma pol- lination was the most appropriate pollination method. Some hvhrids wore nht^inocl ,4~.~ i-~ .1~ ~k L_ _,
出处
《山东林业科技》
2014年第2期21-24,共4页
Journal of Shandong Forestry Science and Technology
关键词
美国红枫
元宝枫
远缘杂交
胚抢救
Acer rubrum
Acer truncatum
distant hybridizations embryo rescue
