摘要
目的研究肺癌患者手术前后血清脂质运载蛋白-2(Lipocalin-2)、基质金属蛋白酶-9(MMP-9)的表达及临床意义。方法采用酶联免疫吸附法(ELISA)检测60例肺癌患者手术前后Lipocalin-2、MMP-9蛋白水平,分析其与临床病理特征的关系。结果肺癌组Lipocalin-2、MMP-9表达水平显著高于健康对照组(P<0.05)。二者表达与TNM临床分期、淋巴结转移状况有关(P<0.05),且呈正相关(r=7.217,P<0.05)。手术治疗总有效率为80.0%,治疗后Lipocalin-2、MMP-9水平显著降低,与治疗前比较差异有统计学意义(P<0.05)。结论肺癌组Lipocalin-2、MMP-9异常高表达,这与肺癌侵袭转移过程密切相关。手术治疗可显著降低二者表达水平,对其表达水平的监测可作为判断手术疗效和评估预后的可靠指标。
Obje ctive To investigate the expression and clinical significance of Lipocalin -2 and MMP-9 before and after operation in patients with lung cancer .Methods The serum levels of Lipocalin-2 and MMP-9 before and after operation were detected by ELISA in 60 patients with lung cancer and 50 healthy subjects.The correlation between the expressions and clinical pathological characteristics was analyzed .Results The serum levels of Lipocalin-2 and MMP-9 in patients with lung cancer were significantly higher than those of healthy subjects （ P ＆lt;0.05）.The expression levels of Lipocalin -2 and MMP-9 were closely correlated to clinical staging and lymph node metastasis （ P ＆lt;0.05）,and the expression levels of Lipocalin-2 were positively related to those of MMP-9 （ r =7.217, P ＆lt;0.05）.The total effective rate of operation was 80.0%.The serum levels of Lipocalin-2 and MMP-9 were significantly decreased after operation ,as compared with those before operation （ P ＆lt;0.05）.There were significant differences in the serum levels of Lipocalin -2,MMP-9 betweem the patients with relapse and the patients without relapse （ P ＆lt;0.05）.C onclusion The overexpression of Lipocalin-2 and MMP-9 is closely related with invasion and metastasis process of lung cancer ,which may play an important role in carcinogenesis ,invasion and metastasis of lung cancer.After operation,the Lipocalin-2 and MMP-9 levels are significantly decreased,and the detection of serum levels of Lipocalin-2 and MMP-9 may be regarded as reliable indexes for evaluating operation efficiency and patient ＆#39;s prognosis.
出处
《河北医药》
CAS
2014年第9期1288-1290,共3页
Hebei Medical Journal
