摘要
目的探讨儿童腺样体肥大(adenoid vegetation)患者血清中肿瘤坏死因子-α(TNF-α)、白介素-4(IL-4)、嗜酸细胞阳离子蛋白(ECP)水平变化及与其肥大程度、体质量指数之间的关系。方法经鼻咽部X线侧位片及查体确诊的87例单纯腺样体肥大患者(实验组)及49例体检正常儿童(对照组),测定其身高、体质量,计算体质量指数(BMI)即体质量/身高2,用腺样体与鼻咽部口径的比值(A/N)来代表腺样体的相对大小;清晨抽取空腹静脉血,应用酶联免疫法(ELISA)测定血清中TNF-α、IL-4水平及Pharmacia CAP System ECP FEIA荧光酶标法测定血清中ECP水平,分析腺样体肥大程度、BMI与血清中TNF-α、IL-4、ECP水平之间的关系,采用SPASS 17.0软件进行统计学分析。结果实验组的血清TNF-α水平为(492.50±13.24)mg/L,与对照组(310.25±10.32)mg/L相比,差异具有统计学意义(P<0.05);实验组与对照组血清IL-4水平分别为(252.91±14.31)pg/mL、(174.37±11.23)pg/mL,差异有统计学意义(P<0.05);ECP水平分别为(6.12±1.18)μg/L、(5.56±0.87)μg/L,差异无统计学意义(P>0.05)。TNF-α水平与腺样体肥大程度分别呈正相关(轻度:r=0.98,P<0.05;中度:r=0.92,P<0.05;重度:r=0.94,P<0.05);IL-4水平与其肥大程度亦分别呈正相关(轻度:r=0.95,P<0.05;中度:r=0.94,P<0.05;重度:r=0.99,P<0.05),且均与BMI无关;ECP水平在两组检测结果中无明显差别(P>0.05)。结论腺样体肥大患者血清中TNF-α、IL-4水平存在不同程度的升高且其水平与腺样体肥大程度呈正相关,与BMI无关;而ECP与腺样体肥大与否及其肥大程度无明显相关性。
Objective To explore the relationship between serum levels of tumor necrosis factor-α （TNF-α）, interleukin-4 （IL-4）, eosinophil cationic protein （ECP） and the degree of adenoid vegetation and body mass index （BMI） in children with adenoid vegetation. Methods 87 cases of children with adenoid vegetation confirmed by X-ray imaging of nasopharynx （experimental group） and 49 normal subjects （control group） were enrolled. The height, weight and body mass index （BMI）of 136 children were documented. The size of vegetation was indicated by the ratio between the adenoid and nasopharyngeal diameter（A/N）. With fasting venous blood, the serum levels of TNF-α, IL-4 were deter mined by enzyme-linked immunosorbent assay kit （ELISA） and serum ECP levels were measured by Pharmacia CAP System ECP FEIA. The relationship between the degrees of adenoid vegetation and BMI and the levels of TNF-α, IL-4, ECP were analyzed by SPASS 17.0. Results The serum levels of TNF-α, IL-4 in experimental group were higher than those in controls （P 〈0.05）, but the levels of ECP were similar in the two groups （P 〉0.05）. Levels of TNF-α and the degree of adenoid vegetation were positively correlated （ mild degree： r = 0. 98, P 〈 0.05 ; mediate degree： r = 0. 92, P 〈 0.05 ; severe degree： r = 0. 9, P 〈 0.05 ） ; Levels of IL-4, but not ECP, were also corelated pos itively with the degree of adenoid vegetation （ mild degree： r = 0. 95, P 〈 0.05 ; mediate degree： r = 0. 94, P 〈 0.05 ; severe degree ： r = 0. 99, P 〈 0.05 ） . Conclusion The serum levels of TNF-α, IL-4 in children with adenoid vegetation increase to some extent, and their levels are corelated positively with the degree of adenoid vegetation as reflected by A/N ratio. , but not with BMI. However, the serum levels of ECP show no obvious correlation.
出处
《山东大学耳鼻喉眼学报》
CAS
2014年第2期58-61,共4页
Journal of Otolaryngology and Ophthalmology of Shandong University
关键词
腺样体肥大
炎症介质
细胞因子
儿童
Adenoid vegetation
Inflammatory mediators
Cyokines
Child
