摘要
目的探讨细胞间黏附分子1(ICAM-1)、血管细胞黏附分子1(VCAM-1)与兔动脉粥样硬化斑块内血管新生的相关性。方法 25只纯种雄性新西兰大白兔给予球囊拉伤及1%胆固醇饲料喂养建立腹主动脉动脉粥样硬化模型,分别于建模后第4、6、8、10、12周末随机选取5只实验兔行安乐死,取腹主动脉组织脱水,包埋制石蜡切片。石蜡切片进行HE染色以及免疫组织化学染色,分析小鼠抗兔巨噬细胞抗体11、ICAM-1、VCAM-1、血小板内皮细胞黏附分子1的表达。结果从4周到12周,斑块体积逐渐增大,巨噬细胞数量、ICAM-1与VCAM-1的表达量也逐渐增加。通过HE染色与血小板内皮细胞黏附分子1标记,第8周斑块内可见新生血管出现,并且新生血管的数量在第10周与第12周逐渐增多。相关性分析显示ICAM-1与VCAM-1的表达与斑块内的血管新生密切相关。结论 ICAM-1、VCAM-1与斑块内血管新生密切相关,它们促进了动脉粥样硬化斑块的进展和不稳定。
Aim To investigate the relationships between intercellular adhesion molecule-1 （ ICAM-1 ）, vascular cell adhesion molecule-1 （ VCAM-1 ）, and intraplaque angiogenesis. Methods Twenty-five New Zealand white rab- bits underwent balloon-induced abdominal aortic wall injury and were fed with a diet of 1% cholesterol to establish a rabbit model of atherosclerosis. In week 4, 6, 8, 10 and 12 after balloon injury every 5 rabbits were euthanasia respectively. Tissue samples were taken from the abdominal aorta. Some segments were embedded in paraffin and cut into 5 μm thick sections for staining with H＆E and reacted with platelet-endothelial cell adhesion molecule-1 （PECAM-1, i.e. CD31 ）, rat anti-rabbit macrophage antibody-11 （ RAM-11 ）, ICAM-1, and VCAM-1. Results From week 4 to week 12, the ex- pression of ICAM-1 and VCAM-1 increased with the development of atherosclerotic plaque in the abdominal aorta of rabbits. Intraplaque neovessels appeared in week 8. There were positive correlations between ICAM-1, VCAM-1, and intraplaque angiogenesis. The correlation between ICAM-1, VCAM-1 and RAM-11 were also positive. Conclusion ICAM-1 and VCAM-! could induce intraplaque angiogenesis, and therefore they play important roles in the development and vulnerabili- ty of atherosclerotic plaques in rabbits.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2014年第3期217-222,共6页
Chinese Journal of Arteriosclerosis
基金
国家973项目基金(2012CB518603)
国家自然科学基金(81270351
60971023)
