摘要
为利用分子标记辅助选择技术选育抗旱高产小麦品种提供基础,以小麦‘旱选10号’/‘鲁麦14＇DH群体为材料,在干旱胁迫及正常灌溉两种水分条件下于2010年和2011年考察小麦主茎结实小穗数,通过采用基于混合线性模型的复合区间作图法分析其QTL,研究控制小麦结实小穗数的数量性状基因.共检测到7个加性QTLs和2对上位性QTLs.加性QTL的LOD值介于2.56～5.08之间,分别位于2D、4A、6A和6B染色体上,对表型变异的贡献率为7.19％～13.65％;上位性QTL的LOD值分别为5.24和5.47,分别位于3A和6B与4B和5B染色体上,对表型贡献率分别为13.71％和17.93％.其中QFns-6A-2于2010年正常灌溉和2011年2种水分条件下均被检测到,可用于分子标记辅助育种.
In order to provide a basis of breeding drought-resistant and high-yielding wheat valneues using marker-assisted selection technique, fertile spikelet number of main stem was investigated in a doubled haploid population derived from two common wheat varieties ＇Hanxuan 10＇ and ＇ Lumai 14＇ in drought stress and normal irrigation conditions in 2010 and 2011 for analyzing quantitative trait loci （QTL） using composite interval mapping method based on mixed linear model. Seven addictive QTLs for fertile spikelet number with LOD scores ranging from 2.56 to 5.08 were detected on 2D, 4A, 6A, and 6B chromosomes respectively, and the phenotypic variations explained by each QTL ranged from 7.19% to 13.65%. Two pairs of epistatic QTLs with LOD scores of 5.24 and 5.47 were detected on 3A-6B and 4B-5B chromosomes, and the phenotypic variations explained by each pair of epistatic QTLs were 13.71% and 17.93%. Among QTLs detected the QFns-6A-2 was detected under normal irrigation condition in 2010 and under two water regimes in 2011, and can be used in marker-assisted breeding.
出处
《农学学报》
2014年第4期5-8,13,共5页
Journal of Agriculture
基金
山西省留学基金项目"小麦优异育种群体的构建及新品种选育"(2010048)
CGIAR挑战计划"Wheat breeding and selection strategies to combine and validate QTLs for WUE and heat tolerance in China"(G7010.02.01-7)
教育部博导类联合项目"小麦持绿性QTL与互作机制"(20121403110005)
关键词
小麦
结实小穗数
DH群体
QTL
Wheat
Fertile Spikelet Number
Doubled Haploid Population
QTL
