摘要
目的:研究蠲痹颗粒抗炎作用的药效学,揭示其抗炎作用的机制,阐明吴氏学派应用扶阳理论治疗痹证(风寒湿痹)临床中药优效的科学基础。方法:建立角叉菜胶致大鼠急性足跖肿胀炎症模型和棉球异物植入诱导大鼠肉芽组织增生慢性炎症模型,随机分为模型对照组、阳性药地塞米松组(2.5 mg·kg-1)、蠲痹颗粒低、中、高剂量组(5.85,11.70,23.40 g·kg-1)。药物干预后,检测蠲痹颗粒灌胃给药对急、慢性炎症反应的影响;建立牛Ⅱ型胶原诱导的DBA/1小鼠胶原关节炎(collagen induced arthritis,CIA)模型,石蜡切片HE染色分析CIA小鼠关节炎病理损伤程度,采用流式微珠阵列法(Cytometric Bead Array,CBA)检测胶原诱导的脾淋巴细胞炎症因子白介素-6(IL-6),肿瘤坏死因子-α(TNF-α),白介素-17A(IL-17A)表达水平。结果:与模型组比较,阳性药组、蠲痹颗粒高剂量组对大鼠角叉菜胶所致足跖肿胀和棉球异物植入引起的大鼠慢性炎症性肉芽组织增生均有显著抑制作用,差异有显著性意义(P<0.05),同时明显改善CIA小鼠局部关节炎的症状,减轻炎症细胞浸润。与正常对照组比较,CIA模型脾淋巴细胞IL-6,TNF-α,IL-17A表达水平显著增加(P<0.01),蠲痹颗粒药物干预后,IL-17A的产生水平显著下调(P<0.05),对炎症因子IL-6,TNF-α无明显影响。结论:蠲痹颗粒具有显著的抗炎作用,其作用机制与抑制促炎因子IL-17A产生有关。
Objective： To investigate anti-inflammatory of Juanbi particle and its relative mechanisms, to clarify the scientific basis of superior efficiency of Chinese medicine in the clinical treatment on Bi Syndrome （wind-cold-dampness Bi） according to Wu School based on strengthening yang theory. Method： The model of carrageenan-induced edema of rat hind and formation of cotton ball granulom in rats were adopted to investigate anti-inflammatory of Juanbi particle, rats were divided into five groups, including the model group,the positive control group（dexamethasone, Dex, 2.5 mg·kg^-1）, Juanbi particle group（5.85,11.70,23.40 g·kg^-1）.Type Ⅱ bovine collagen（CⅡ）-induced arthritis （CIA） in DBA/1 mice were established for investigating the antiarthritic potential of Juanbi particle. The histological damage was assessed by histological examination （HE）, the inflammatory cytokine production levels of interleukin（IL）-6,IL-17A and tumor necrosis factor-α（TNF-α） in CⅡ-induced splenic lymphocytes was detected by cytometric bead array （CBA）. Result： Compared with the model group,high dosage group of Juanbi particle and positive control group were significantly inhibited swelling of paw and the formation of cotton ball granuloma（P〈0.05）; meanwhile the clinical score and inflammatory cells infiltration in CIA mice were significantly reduced in the Juanbi particle administration group. Compared with the normal group,there are significantly increased the inflammatory cytokine level of IL-17A ,IL-6 and TNF-α induced by CⅡ （P〈0.01）. Compared with the model group ,the inflammatory cytokine level of IL-17A was reduced by the Juanbi particle （P〈0.05）, while the IL-6 and TNF-α have no significant changed. Conclusion： Juanbi particle has an anti-inflammatory effect, the mechanism of which may be related to suppress inflammatory cytokine production levels of IL-17A.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第10期117-121,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81360543)
云南省科技计划重点新产品开发计划项目(2012BCO10)
云南省自然科学基金青年项目(2012FD097)
