摘要
目的观察消微防石汤对大鼠肾脏草酸钙微结石防治作用并初步探讨其防治机制。方法 72只Wistar大鼠按随机数字表法分成6组,即正常组、生理盐水阴性对照组、复方金钱草颗粒阳性对照组、消微防石汤低、中、高剂量实验组。正常组不予以任何处理,其它各组均上午给予乙二醇以造模、下午分别给予生理盐水、复方金钱草颗粒溶化液及低、中、高剂量消微防石汤,连续灌药5天。第5天末次灌药后各组均收集12小时尿液离心取清液并检测尿液pH值及草酸、钙、肌酐浓度,称各组大鼠体重并腹腔麻醉,采腹主动脉血离心取血清并检测动脉血清草酸、钙、肌酐浓度,取肾组织称重并制作切片观察肾组织成石程度。结果六组大鼠肾组织总体成石率差异有统计学意义(P<0.01),与生理盐水阴性对照组比较,消微防石汤中、高剂量实验组差异均有统计学意义(P<0.05、P<0.01),复方金钱草颗粒阳性对照组、消微防石汤低剂量实验组差异均无统计学意义(均为P>0.05),与复方金钱草颗粒阳性对照组比较,消微防石汤低、中、高剂量实验组差异均无统计学意义(均为P>0.05);六组大鼠尿液总体pH值、动脉血清与尿液中草酸、钙、肌酐总体浓度差异均无统计学意义(均为P>0.05),消微防石汤中、高剂量实验组分别与正常组、生理盐水阴性对照组比较,尿液pH值、动脉血清与尿液中草酸、钙、肌酐浓度差异均无统计学意义(均为P>0.05);六组大鼠左肾重量、右肾重量、终末体重总体差异分别有统计学意义(P<0.05、P<0.01、P<0.01)。结论消微防石汤对大鼠肾脏草酸钙微结石具有防治作用,作用机制可能是通过影响草酸钙晶体的附着、排出使得肾组织草酸钙晶体减少,而不是通过影响大鼠尿液pH值及动脉血清与尿液中草酸、钙浓度以影响草酸钙晶体的形成。
Objective To observe the precautionary and therapeutic effects of Xiaowei Fangshi Decoction on the micro calcium oxalate kidney stones of rats and to explore its mechanisms preliminarily. Methods 72 Wistar rats were divided randomly by random digits table into the normal group, the saline negative control group, the Compound Loosestrife Particles positive control group, the low dose Xiaowei Fangshi Decoction experimental group, the medium dose Xiaowei Fangshi Decoction experimental group, the high dose Xiaowei Fangshi Decoction experimental group. Except those in the normal group, rats in the rest groups received Glycol every morning for 5 consecutive days. In addition, the saline negative control group, the Compound Loosestrife Particles positive control group, the low dose Xiaowei Fangshi Decoction experimental group, the medium dose Xiaowei Fangshi Decoction experimental group, the high dose Xiao-wei Fangshi Decoction experimental group received normal saline, Compound Loosestrife Particles Solution, the low dose Xiaowei Fangshi Decoction, the medium dose Xiaowei Fangshi Decoction, the high dose Xiao-wei Fangshi Decoction respectively every afternoon for 5 consecutive days. After the last drench on the fifth＆nbsp;day, the rats in all groups were collected 12h urine, weighed, drawed abdominal aortic blood and taken kidneys. Each group of urine was taken the supernatant after centrifugation, and then measured pH, oxalic acid, calcium, creatinine. Each group of abdominal aortic blood was taken the serum after centrifugation, and then measured oxalic acid, calcium, creatinine. Each group of kidneys was weighted, and then made into tissue slices. Results The extent of stone in the six groups was statistically significant （P0. 05）, but the medium dose Xiaowei Fangshi Decoction experimental group and the high dose Xiaowei Fangshi Decoction experimental group were statistically significant respectively（ P0. 05）. The overall differences of the pH in the urine, the oxalic acid, calcium, creati-nine in the urin
出处
《环球中医药》
CAS
2014年第4期266-271,共6页
Global Traditional Chinese Medicine
关键词
早期尿石病诊断法
泌尿系结石
中医药
实验研究
Early diagnosis of urinary stone disease
Urinary stones
Traditional Chinese Medicine
Experimental research
