摘要
目的探讨利用酶联免疫斑点(Elispot)法检测胸水结核分枝杆菌特异性IFN-γ在诊断结核性胸膜炎的价值。方法对147例结核性胸膜炎患者(结核性胸膜炎组)、69例非结核性胸膜炎患者(对照组)胸水单个核细胞结核菌抗原特异性IFN-γ分泌水平进行检测,对比两者的检测差异。结果结核性胸膜炎组患者胸水IFN-γ水平显著高于对照组(P<0.001)。结论利用酶联免疫斑点技术检测胸液标本结核分枝杆菌特异性IFN-γ可用于结核性胸膜炎的诊断及鉴别诊断。
Objective To analyze the diagnostic value of detection of IFN-γ, in pleural effusion to tubercu- lous pleuritis. Methods The level of IFN-γ was detected in 147 patients with tuberculous pleuritis and 69 patients with non-tuberculous pleuritis by Elispot technology. Results The level of IFN-γ, in patients with tuberculous pleuri- tis was significantly higher than that in patients with non-tuberculous pleufitis （ P 〈 0. 001 ）. Conclusion The detec- tion of mycobacterium tuberculosis-specific interferon-gamma in pleural effusion by Enzyme-linked immunospot assay is useful for diagnosis of patients with tuberculous pleuritis.
出处
《临床肺科杂志》
2014年第6期1064-1066,共3页
Journal of Clinical Pulmonary Medicine
关键词
酶联免疫斑点技术
结核性胸膜炎
Γ干扰素
Enzyme-linked immunospot assay
tuberculous pleuritis
interferon-gamma
