摘要
稻瘟病是严重威胁水稻种植生产的三大主要病害之一。采用抗稻瘟病的野生稻种质材料为供体亲本,与栽培稻测253和GX-12为轮回亲本,通过多代杂交、回交和自交,培育出6个野生稻渗入系群体。经过自然诱发鉴定和苗期人工鉴定,分别从野生稻渗入系群体C1、C2和G1筛选出69份抗稻瘟病材料。其中,抗稻瘟病生理小种ZA1的渗入系材料有38份,抗ZB17小种21份,抗ZC13小种10份。随后,以对ZA1小种表现抗性的C1选择群体为定位群体,运用基于"遗传搭车"原理的卡方分析法挖掘稻瘟病抗性基因/QTL位点,分别在1、2、5、6号染色体上检测到11个抗病QTLs。研究结果可为今后水稻抗病新品种选育提供重要的基因材料和技术理论依据。
Rice blast is a serious threat to rice production as one of the 3 major diseases.In this study,6 groups of wild rice introgression lines were obtained by multiple generation selection of hybrids and backcrossing with wild rice germplasm resistant to rice blast as donor parents and cultivated rice Ce253 and GX-12 as recurrent parents.69 blast resistant materials were selected from the 3 wild rice introgreasion lines,namely C1,C2 and G1 after natural induction and artificial identification during seedling period.Among them,38 accessions were blast resistant to race ZA1,21 accessions were resistant to race ZB17 and 10 accessions were resistant to race ZC13.Using Chi-square analysis based on‘ Genetic hitchhiking＇ principle and mapping population C1 resistant to race ZA1,11 QTLs were detected on chromosome 1,2,5 and 6.This result would provide important genetic material and technical theory for disease resistance in rice breeding in future.
出处
《西南农业学报》
CSCD
北大核心
2014年第2期613-619,共7页
Southwest China Journal of Agricultural Sciences
基金
国家自然基金项目(31260318)
广西科学研究与技术开发计划项目(桂科攻1123001-3A)
关键词
野生稻渗入系
稻瘟病
鉴定
QTL
Wild rice introgression line
Rice blast
Identification
QTL
